製品: hnRNP Q Antibody
カタログ: DF3031
タンパク質の説明: Rabbit polyclonal antibody to hnRNP Q
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 62 KD; 70kD(Calculated).
ユニプロット: O60506
RRID: AB_2835391

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
hnRNP Q Antibody detects endogenous levels of total hnRNP Q.
RRID:
AB_2835391
引用形式: Affinity Biosciences Cat# DF3031, RRID:AB_2835391.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

cytoplasmic RNA-interacting protein; dJ3J17.2; Glycine and tyrosine rich RNA binding protein; Glycine- and tyrosine-rich RNA-binding protein; GRY RBP; GRY-RBP; GRYRBP; Heterogeneous nuclear ribonucleoprotein Q; hnRNP Q; HNRPQ; HNRPQ_HUMAN; HNRPQ1; NS1 associated protein 1; NS1-associated protein 1; NSAP1; pp68; RP1 3J17.2; Synaptotagmin binding cytoplasmic RNA interacting protein; Synaptotagmin-binding; Syncrip;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O60506 HNRPQ_HUMAN:

Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.

タンパク質配列:
MATEHVNGNGTEEPMDTTSAVIHSENFQTLLDAGLPQKVAEKLDEIYVAGLVAHSDLDERAIEALKEFNEDGALAVLQQFKDSDLSHVQNKSAFLCGVMKTYRQREKQGTKVADSSKGPDEAKIKALLERTGYTLDVTTGQRKYGGPPPDSVYSGQQPSVGTEIFVGKIPRDLFEDELVPLFEKAGPIWDLRLMMDPLTGLNRGYAFVTFCTKEAAQEAVKLYNNHEIRSGKHIGVCISVANNRLFVGSIPKSKTKEQILEEFSKVTEGLTDVILYHQPDDKKKNRGFCFLEYEDHKTAAQARRRLMSGKVKVWGNVGTVEWADPIEDPDPEVMAKVKVLFVRNLANTVTEEILEKAFSQFGKLERVKKLKDYAFIHFDERDGAVKAMEEMNGKDLEGENIEIVFAKPPDQKRKERKAQRQAAKNQMYDDYYYYGPPHMPPPTRGRGRGGRGGYGYPPDYYGYEDYYDYYGYDYHNYRGGYEDPYYGYEDFQVGARGRGGRGARGAAPSRGRGAAPPRGRAGYSQRGGPGSARGVRGARGGAQQQRGRGVRGARGGRGGNVGGKRKADGYNQPDSKRRQTNNQNWGSQPIAQQPLQGGDHSGNYGYKSENQEFYQDTFGQQWK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60506 基板として

Site PTM Type Enzyme
A2 Acetylation
Y47 Phosphorylation
S55 Phosphorylation
K66 Ubiquitination
K81 Acetylation
K81 Ubiquitination
K91 Ubiquitination
S92 Phosphorylation
K100 Acetylation
K100 Ubiquitination
K107 Ubiquitination
T110 Phosphorylation
K111 Ubiquitination
S115 Phosphorylation
S116 Phosphorylation
K117 Ubiquitination
K123 Acetylation
K123 Methylation
K123 Ubiquitination
K125 Methylation
Y133 Phosphorylation
T138 Phosphorylation
Y144 Phosphorylation
S159 Phosphorylation
K168 Ubiquitination
K184 Ubiquitination
R192 Methylation
K213 Methylation
K221 Acetylation
K221 Ubiquitination
S249 Phosphorylation
T255 Phosphorylation
K256 Ubiquitination
K265 Ubiquitination
Y276 Phosphorylation
K282 Acetylation
K282 Ubiquitination
K283 Acetylation
K283 Ubiquitination
K284 Ubiquitination
R286 Methylation
Y293 Phosphorylation
K297 Sumoylation
K297 Ubiquitination
K310 Ubiquitination
K312 Ubiquitination
K336 Ubiquitination
K338 Acetylation
K338 Ubiquitination
T348 Phosphorylation
K356 Ubiquitination
K363 Acetylation
K363 Methylation
K363 Ubiquitination
K371 Ubiquitination
Y373 Phosphorylation P11362 (FGFR1)
R381 Methylation
K386 Ubiquitination
K407 Ubiquitination
K412 Acetylation
K412 Ubiquitination
Y431 Phosphorylation
Y432 Phosphorylation
Y434 Phosphorylation
R444 Methylation
R446 Methylation
R448 Methylation
Y472 Phosphorylation
Y474 Phosphorylation
R478 Methylation
Y485 Phosphorylation
Y488 Phosphorylation
R496 Methylation
R498 Methylation
R501 Methylation
R504 Methylation
S509 Phosphorylation
R510 Methylation
R518 Methylation
R520 Methylation
S524 Phosphorylation
R526 Methylation
S531 Phosphorylation
R533 Methylation
R536 Methylation
R539 Methylation
R565 Methylation
K566 Acetylation
K566 Ubiquitination
Y570 Phosphorylation
S575 Phosphorylation
K576 Acetylation
K576 Ubiquitination
T580 Phosphorylation
S587 Phosphorylation
S601 Phosphorylation
Y614 Phosphorylation
K623 Acetylation

研究背景

機能:

Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation; seems not to be essential for GAIT complex function.

PTMs:

Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated (By similarity).

細胞の位置付け:

Cytoplasm. Microsome. Endoplasmic reticulum. Nucleus.
Note: The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs (By similarity).

Nucleus>Nucleoplasm.
Note: Expressed predominantly in the nucleoplasm.

Nucleus>Nucleoplasm.
Note: Expressed predominantly in the nucleoplasm.

Nucleus>Nucleoplasm.
Note: Expressed predominantly in the nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.

サブユニット構造:

Isoform 1 is a component of the APOB mRNA editosome complex and interacts with APOBEC1 and A1CF (APOBEC1 complementation factor). Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR, HNRPD and SYNCRIP. Isoform 3 interacts with HNRPR. Interacts with POLR2A hyperphosphorylated C-terminal domain. Isoform 1, isoform 2 and isoform 3 interact with SMN. Isoform 3 interacts through its C-terminal domain with SYT7, SYT8 and SYT9 (By similarity). The non-phosphorylated and phosphorylated forms are colocalized with PAIP1 in polysomes (By similarity). Interacts with HABP4. Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 (By similarity). Identified in the spliceosome C complex. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with GTPBP1. Component of the GAIT complex; in humans the complex assembly seems to be a two-step process in which EPRS1 first associates with SYNCRIP to form a pre-GAIT complex which is deficient in GAIT element binding.

(Microbial infection) Interacts with minute virus of mice (MVM) NS1 protein.

タンパク質ファミリー:

The domain containing eight Arg-Gly-Gly repeats (RGG/RXR-box) may be involved in RNA-binding and protein-protein interactions. It is methylated by PRMT1, and essential for nuclear localization.

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