AFfirm™ AATF Mouse Monoclonal Antibody - #BF9325
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
AATF; AATF_HUMAN; Apoptosis antagonizing transcription factor; Apoptosis-antagonizing transcription factor; BFR2; CHE 1; CHE1; DED; Protein AATF; Rb binding protein Che 1; Rb-binding protein Che-1;
免疫原
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
- Q9NY61 AATF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGPQPLALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLTTNQLPQPDVFPLFKDKGGPEFSSALKNSHKALKALLRSLVGLQEELLFQYPDTRYLVDGTKPNAGSEEISSEDDELVEEKKQQRRRVPAKRKLEMEDYPSFMAKRFADFTVYRNRTLQKWHDKTKLASGKLGKGFGAFERSILTQIDHILMDKERLLRRTQTKRSVYRVLGKPEPAAQPVPESLPGEPEILPQAPANAHLKDLDEEIFDDDDFYHQLLRELIERKTSSLDPNDQVAMGRQWLAIQKLRSKIHKKVDRKASKGRKLRFHVLSKLLSFMAPIDHTTMNDDARTELYRSLFGQLHPPDEGHGD
PTMs - Q9NY61 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K58 | Sumoylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| Y117 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| S143 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K145 | Ubiquitination | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S203 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| K223 | Sumoylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K276 | Methylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| Y305 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K342 | Sumoylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| T366 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| K369 | Acetylation | Uniprot | |
| K380 | Acetylation | Uniprot | |
| K383 | Acetylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| K422 | Sumoylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| K475 | Sumoylation | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| T476 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K496 | Ubiquitination | Uniprot | |
| S510 | Phosphorylation | O96017 (CHEK2) | Uniprot |
研究背景
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Hyperphosphorylated during the G1/S phase transition.
Nucleus>Nucleolus.
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT.
Belongs to the AATF family.
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