AFfirm™ WTAP Mouse Monoclonal Antibody - #BF9330
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
DKFZp686F20131; Female-lethal(2)D homolog; FL2D_HUMAN; hFL(2)D; KIAA0105; MGC3925; Mum2; OTTHUMP00000017522; OTTHUMP00000017523; PNAS 132; PNAS-132; PNAS132; Pre mRNA splicing regulator WTAP; Pre-mRNA-splicing regulator WTAP; Putative pre mRNA splicing regulator female lethal 2D homolog; Putative pre mRNA splicing regulator female lethal(2D); Putative pre-mRNA splicing regulator female lethal(2D) homolog; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; Wilms tumor 1 associating protein; Wilms tumor 1-associating protein; Wilms' tumor 1 associating protein; Wilms' tumour 1-associating protein; WT1 associated protein; WT1-associated protein; WT1-associating protein; wtap;
免疫原
- Q15007 FL2D_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTNEEPLPKKVRLSETDFKVMARDELILRWKQYEAYVQALEGKYTDLNSNDVTGLRESEEKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLKETRQQLAQYQQQQSQASAPSTSRTTASEPVEQSEATSKDCSRLTNGPSNGSSSRQRTSGSGFHREGNTTEDDFPSSPGNGNKSSNSSEERTGRGGSGYVNQLSAGYESVDSPTGSENSLTHQSNDTDSSHDPQEEKAVSGKGNRTVGSRHVQNGLDSSVNVQGSVL
PTMs - Q15007 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T2 | Phosphorylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K125 | Sumoylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| C270 | S-Nitrosylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| T299 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot |
研究背景
Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.
Nucleus speckle. Nucleus>Nucleoplasm. Cytoplasm.
Note: Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus.
Ubiquitously expressed.
Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM. The MAC subcomplex is composed of METTL3 and METTL14. The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B). Interacts with WT1. Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3.
Belongs to the fl(2)d family.
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