AFfirm™ EHHADH Mouse Monoclonal Antibody - #BF9339
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
3 hydroxyacyl CoA dehydrogenase; 3,2 trans enoyl CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; ECHD; ECHP_HUMAN; EHHADH; Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase; L 3 hydroxyacyl CoA dehydrogenase; L bifunctional protein, peroxisomal; L PBE; LBFP; LBP; MGC120586; MS730; PBE; PBFE; Peroxisomal bifunctional enzyme; Peroxisomal enoyl CoA hydratase;
免疫原
Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.
- Q08426 ECHP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEYTRLHNALALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHTIKAIVICGAEGKFSAGADIRGFSAPRTFGLTLGHVVDEIQRNEKPVVAAIQGMAFGGGLELALGCHYRIAHAEAQVGLPEVTLGLLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLGILDKVVNSDPVEEAIRFAQRVSDQPLESRRLCNKPIQSLPNMDSIFSEALLKMRRQHPGCLAQEACVRAVQAAVQYPYEVGIKKEEELFLYLLQSGQARALQYAFFAERKANKWSTPSGASWKTASARPVSSVGVVGLGTMGRGIVISFARARIPVIAVDSDKNQLATANKMITSVLEKEASKMQQSGHPWSGPKPRLTSSVKELGGVDLVIEAVFEEMSLKKQVFAELSAVCKPEAFLCTNTSALDVDEIASSTDRPHLVIGTHFFSPAHVMKLLEVIPSQYSSPTTIATVMNLSKKIKKIGVVVGNCFGFVGNRMLNPYYNQAYFLLEEGSKPEEVDQVLEEFGFKMGPFRVSDLAGLDVGWKSRKGQGLTGPTLLPGTPARKRGNRRYCPIPDVLCELGRFGQKTGKGWYQYDKPLGRIHKPDPWLSKFLSRYRKTHHIEPRTISQDEILERCLYSLINEAFRILGEGIAASPEHIDVVYLHGYGWPRHKGGPMFYASTVGLPTVLEKLQKYYRQNPDIPQLEPSDYLKKLASQGNPPLKEWQSLAGSPSSKL
PTMs - Q08426 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T26 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| K165 | Acetylation | Uniprot | |
| K171 | Acetylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| K219 | Acetylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| K280 | Acetylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| K330 | Acetylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| K346 | Acetylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K464 | Acetylation | Uniprot | |
| K532 | Acetylation | Uniprot | |
| T548 | Phosphorylation | Uniprot | |
| K577 | Acetylation | Uniprot | |
| Y580 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| K584 | Acetylation | Uniprot | |
| K598 | Acetylation | Uniprot | |
| Y666 | Phosphorylation | Uniprot | |
| Y682 | Phosphorylation | Uniprot | |
| Y683 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| K722 | Acetylation | Uniprot |
研究背景
Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
Peroxisome.
Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.
Monomer.
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
研究領域
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Metabolism > Lipid metabolism > Fatty acid degradation.
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Amino acid metabolism > Lysine degradation.
· Metabolism > Amino acid metabolism > Tryptophan metabolism.
· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Carbohydrate metabolism > Butanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Fatty acid metabolism.
· Organismal Systems > Endocrine system > PPAR signaling pathway.
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