AFfirm™ USP13 Mouse Monoclonal Antibody - #BF9358
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
Deubiquitinating enzyme 13; Isopeptidase T 3; Isopeptidase T-3; Isopeptidase T3; ISOT 3; ISOT-3; ISOT3; Ubiquitin carboxyl terminal hydrolase 13; Ubiquitin carboxyl-terminal hydrolase 13; Ubiquitin specific peptidase 13 (isopeptidase T3); Ubiquitin specific peptidase 13; Ubiquitin specific processing protease 13; Ubiquitin specific protease 13; Ubiquitin thioesterase 13; Ubiquitin thiolesterase 13; Ubiquitin-specific-processing protease 13; UBP13; UBP13_HUMAN; USP 13; USP13;
免疫原
- Q92995 UBP13_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQRRGALFGMPGGSGGRKMAAGDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEGGLYVCMNTFLAFGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDLDTDDDLNSDDYEYEDEAKLVIFPDHYEIALPNIEELPALVTIACDAVLSSKSPYRKQDPDTWENELPVSKYANNLTQLDNGVRIPPSGWKCARCDLRENLWLNLTDGSVLCGKWFFDSSGGNGHALEHYRDMGYPLAVKLGTITPDGADVYSFQEEEPVLDPHLAKHLAHFGIDMLHMHGTENGLQDNDIKLRVSEWEVIQESGTKLKPMYGPGYTGLKNLGNSCYLSSVMQAIFSIPEFQRAYVGNLPRIFDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQQNGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSDVFRFLVEERIQCCQTRKVRYTERVDYLMQLPVAMEAATNKDELIAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS
PTMs - Q92995 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R4 | Methylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| R101 | Methylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| T122 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| T380 | Phosphorylation | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| K405 | Sumoylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K511 | Ubiquitination | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| K598 | Ubiquitination | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| K640 | Sumoylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| K841 | Ubiquitination | Uniprot | |
| K850 | Ubiquitination | Uniprot |
研究背景
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
Highly expressed in ovary and testes.
Interacts with UFD1. Interacts (via UBA domains) with SIAH2 (when ubiquitinated). Interacts with BAG6; the interaction is direct and may mediate UBL4A deubiquitination. Interacts (via UBA 2 domain) with AMFR; the interaction is direct. Interacts with UBL4A; may be indirect via BAG6.
The UBP-type zinc finger has lost its ability to bind ubiquitin and USP13 is not activated by unanchored ubiquitin. Swapping with the UBP-type zinc finger from USP5 restores ability to bind unanchored ubiquitin and subsequent activation of the protein (PubMed:22216260).
The UBA domains mediate binding to ubiquitin.
Belongs to the peptidase C19 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.