製品: GRK6 Antibody
カタログ: DF3180
タンパク質の説明: Rabbit polyclonal antibody to GRK6
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 65 KD; 66kD(Calculated).
ユニプロット: P43250
RRID: AB_2835403

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
GRK6 Antibody detects endogenous levels of total GRK6.
RRID:
AB_2835403
引用形式: Affinity Biosciences Cat# DF3180, RRID:AB_2835403.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FLJ32135; G protein coupled receptor kinase 6; G protein coupled receptor kinase GRK6; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6; Gprk6; Grk6; GRK6_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P43250 GRK6_HUMAN:

Widely expressed.

タンパク質配列:
MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELSRCVAFLDGVAEYEVTPDDKRKACGRQLTQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSERFSPQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQDCCGNCSDSEEELPTRL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43250 基板として

Site PTM Type Enzyme
T10 Phosphorylation
K14 Ubiquitination
T106 Phosphorylation
S111 Phosphorylation
T113 Phosphorylation
K139 Ubiquitination
K183 Ubiquitination
K194 Ubiquitination
K210 Ubiquitination
K226 Ubiquitination
K235 Acetylation
K235 Ubiquitination
K240 Acetylation
K240 Ubiquitination
K313 Ubiquitination
K343 Ubiquitination
K358 Ubiquitination
K402 Ubiquitination
K426 Ubiquitination
K443 Ubiquitination
K466 Ubiquitination
S484 Phosphorylation P43250 (GRK6)
T485 Phosphorylation P43250 (GRK6)
K487 Ubiquitination
S557 Phosphorylation
S566 Phosphorylation
S568 Phosphorylation

PTMs - P43250 酵素として

Substrate Site Source
O14745 (SLC9A3R1) S290 Uniprot
O75581 (LRP6) S1448 Uniprot
O75581 (LRP6) S1490 Uniprot
O75581 (LRP6) S1508 Uniprot
O75581 (LRP6) T1572 Uniprot
O75581 (LRP6) S1590 Uniprot
P07550 (ADRB2) S355 Uniprot
P07550 (ADRB2) S356 Uniprot
P08069 (IGF1R) S1321 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P30411-2 (BDKRB2) S346 Uniprot
P30411-2 (BDKRB2) S348 Uniprot
P30411 (BDKRB2) S366 Uniprot
P30411 (BDKRB2) S373 Uniprot
P30411-1 (BDKRB2) S375 Uniprot
P37840-1 (SNCA) S9 Uniprot
P37840 (SNCA) S129 Uniprot
P43250 (GRK6) S484 Uniprot
P43250-3 (GRK6) T485 Uniprot
P61073 (CXCR4) S324 Uniprot
P61073 (CXCR4) S325 Uniprot
P61073 (CXCR4) S330 Uniprot
P61073 (CXCR4) S339 Uniprot
Q15722 (LTB4R) T308 Uniprot

研究背景

機能:

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).

PTMs:

It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.

細胞の位置付け:

Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

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