AFfirm™ PAM Mouse Monoclonal Antibody - #BF9422
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
AMD_HUMAN; PAL; PAM; Pancreatic peptidylglycine alpha amidating monooxygenase; Peptidyl alpha amidating enzyme; Peptidyl alpha hydroxyglycine alpha amidating lyase; Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; Peptidylamidoglycolate lyase; Peptidylglycine 2 hydroxylase; Peptidylglycine alpha amidating monooxygenase; Peptidylglycine alpha hydroxylating monooxygenase; PHM;
免疫原
- P19021 AMD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGRVPSLLVLLVFPSSCLAFRSPLSVFKRFKETTRPFSNECLGTTRPVVPIDSSDFALDIRMPGVTPKQSDTYFCMSMRIPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPAGEKVVNSDISCHYKNYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIPVKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVLDQGDFYSLLSKLLGEREDVVHVHKYNPTEKAESESDLVAEIANVVQKKDLGRSDAREGAEHERGNAILVRDRIHKFHRLVSTLRPPESRVFSLQQPPPGEGTWEPEHTGDFHMEEALDWPGVYLLPGQVSGVALDPKNNLVIFHRGDHVWDGNSFDSKFVYQQIGLGPIEEDTILVIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDVALHQVFKLDPNNKEGPVLILGRSMQPGSDQNHFCQPTDVAVDPGTGAIYVSDGYCNSRIVQFSPSGKFITQWGEESSGSSPLPGQFTVPHSLALVPLLGQLCVADRENGRIQCFKTDTKEFVREIKHSSFGRNVFAISYIPGLLFAVNGKPHFGDQEPVQGFVMNFSNGEIIDIFKPVRKHFDMPHDIVASEDGTVYIGDAHTNTVWKFTLTEKLEHRSVKKAGIEVQEIKEAEAVVETKMENKPTSSELQKMQEKQKLIKEPGSGVPVVLITTLLVIPVVVLLAIAIFIRWKKSRAFGDSEHKLETSSGRVLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRLSTEGSDQEKEDDGSESEEEYSAPLPALAPSSS
PTMs - P19021 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K69 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| T413 | O-Glycosylation | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| S466 | O-Glycosylation | Uniprot | |
| T467 | O-Glycosylation | Uniprot | |
| S473 | O-Glycosylation | Uniprot | |
| K610 | Ubiquitination | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| Y651 | Phosphorylation | Uniprot | |
| K712 | Ubiquitination | Uniprot | |
| K716 | Ubiquitination | Uniprot | |
| K819 | Ubiquitination | Uniprot | |
| T836 | Phosphorylation | Uniprot | |
| K901 | Ubiquitination | Uniprot | |
| K916 | Ubiquitination | Uniprot | |
| S918 | Phosphorylation | Uniprot | |
| S929 | Phosphorylation | Uniprot | |
| S934 | Phosphorylation | Uniprot | |
| S942 | Phosphorylation | Uniprot | |
| T943 | Phosphorylation | Uniprot | |
| S946 | Phosphorylation | Q8TAS1 (UHMK1) | Uniprot |
| S955 | Phosphorylation | Uniprot | |
| S957 | Phosphorylation | Uniprot |
研究背景
Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (By similarity).
Cytoplasmic vesicle>Secretory vesicle membrane>Single-pass membrane protein.
Note: Secretory granules.
Membrane>Single-pass type I membrane protein.
Membrane>Single-pass type I membrane protein.
Secreted.
Note: Secreted from secretory granules.
Secreted.
Note: Secreted from secretory granules.
Monomer. Interacts with RASSF9.
In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
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