AFfirm™ ENaC gamma Mouse Monoclonal Antibody - #BF9433

製品: ENaC gamma Mouse Monoclonal Antibody
カタログ: BF9433
タンパク質の説明: Mouse monoclonal antibody to ENaC gamma
アプリケーション: WB
反応性: Human
予測: Mouse, Rat, Bovine, Horse, Dog
分子量: 75-95 kDa; 74kD(Calculated).
ユニプロット: P51170

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFfirm9433]
特異性:
ENaC gamma Mouse Monoclonal Antibody detects endogenous levels of total ENaC gamma.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Amiloride sensitive epithelial sodium channel gamma subunit; Amiloride sensitive sodium channel subunit gamma; Amiloride-sensitive sodium channel subunit gamma; BESC3; ENaC gamma subunit; ENaCG; ENaCgamma; Epithelial Na(+) channel subunit gamma; Epithelial Na+ channel subunit gamma; Gamma ENaC; Gamma NaCH; Gamma-ENaC; Gamma-NaCH; Nonvoltage gated sodium channel 1 subunit gamma; Nonvoltage-gated sodium channel 1 subunit gamma; PHA 1; PHA1; SCNEG; SCNN 1G; SCNN1G; SCNNG_HUMAN; Sodium channel non voltage gated 1 gamma subunit; Sodium channel nonvoltage gated 1 gamma;

免疫原

免疫原:

A synthesized peptide derived from human ENaC gamma, corresponding to a region within C-terminal amino acids.

Uniprot:

P51170(SCNNG_HUMAN) >>Visit HPA database.

遺伝子(ID):
SCNN1G(6340)
発現特異性:
P51170 SCNNG_HUMAN:

Expressed in kidney (at protein level).

タンパク質配列:
MAPGEKIKAKIKKNLPVTGPQAPTIKELMRWYCLNTNTHGCRRIVVSRGRLRRLLWIGFTLTAVALILWQCALLVFSFYTVSVSIKVHFRKLDFPAVTICNINPYKYSTVRHLLADLEQETREALKSLYGFPESRKRREAESWNSVSEGKQPRFSHRIPLLIFDQDEKGKARDFFTGRKRKVGGSIIHKASNVMHIESKQVVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAEELLVTCFFDGVSCDARNFTLFHHPMHGNCYTFNNRENETILSTSMGGSEYGLQVILYINEEEYNPFLVSSTGAKVIIHRQDEYPFVEDVGTEIETAMVTSIGMHLTESFKLSEPYSQCTEDGSDVPIRNIYNAAYSLQICLHSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLHRAFVQEELGCQSVCKEACSFKEWTLTTSLAQWPSVVSEKWLLPVLTWDQGRQVNKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQWQKAKEWWAWKQAPPCPEAPRSPQGQDNPALDIDDDLPTFNSALHLPPALGTQVPGTPPPKYNTLRLERAFSNQLTDTQMLDEL

研究背景

機能:

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

PTMs:

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.

Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.

ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.

細胞の位置付け:

Apical cell membrane>Multi-pass membrane protein.
Note: Apical membrane of epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in kidney (at protein level).

タンパク質ファミリー:

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.

研究領域

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

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