HOXA1 Antibody - #DF3187

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製品: HOXA1 Antibody
カタログ: DF3187
タンパク質の説明: Rabbit polyclonal antibody to HOXA1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 37 KD; 37kD(Calculated).
ユニプロット: P49639
RRID: AB_2835410

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
HOXA1 Antibody detects endogenous levels of total HOXA1.
RRID:
AB_2835410
引用形式: Affinity Biosciences Cat# DF3187, RRID:AB_2835410.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BSAS; Homeo box A1; Homeobox 1F; Homeobox A1; Homeobox protein Hox A1; Homeobox protein Hox-1F; Homeobox protein Hox-A1; Hox 1.6 like protein; Hox 1F; HOX A1; HOX A1 homeodomain protein; HOX1; HOX1F; hoxa1; hoxb1b; HXA1_HUMAN; Lab like protein; MGC45232;

免疫原

免疫原:
Uniprot:

P49639(HXA1_HUMAN) >>Visit HPA database.

遺伝子(ID):
HOXA1(3198)
タンパク質配列:
MDNARMNSFLEYPILSSGDSGTCSARAYPSDHRITTFQSCAVSANSCGGDDRFLVGRGVQIGSPHHHHHHHHHHPQPATYQTSGNLGVSYSHSSCGPSYGSQNFSAPYSPYALNQEADVSGGYPQCAPAVYSGNLSSPMVQHHHHHQGYAGGAVGSPQYIHHSYGQEHQSLALATYNNSLSPLHASHQEACRSPASETSSPAQTFDWMKVKRNPPKTGKVGEYGYLGQPNAVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNRRMKQKKREKEGLLPISPATPPGNDEKAEESSEKSSSSPCVPSPGSSTSDTLTTSH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49639 基板として

Site PTM Type Enzyme
Y28 Phosphorylation
S30 Phosphorylation
T255 Phosphorylation
S296 Phosphorylation
T299 Phosphorylation
S334 Phosphorylation

研究背景

機能:

Sequence-specific transcription factor (By similarity). Regulates multiple developmental processes including brainstem, inner and outer ear, abducens nerve and cardiovascular development and morphogenesis as well as cognition and behavior. Also part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments (By similarity). Activates transcription in the presence of PBX1A and PKNOX1 (By similarity).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with OGT (via TPR repeats domain); the interaction takes place mainly in the nucleus.

タンパク質ファミリー:

Belongs to the Antp homeobox family. Labial subfamily.

参考文献

1). Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway. eBioMedicine, 2022 (PubMed: 35525203) [IF=11.1]
2). HOXA1 participates in VSMC-to-macrophage-like cell transformation via regulation of NF-κB p65 and KLF4: a potential mechanism of atherosclerosis pathogenesis. Molecular Medicine, 2023 (PubMed: 37528397) [IF=5.7]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 1 HOXA1 expression is increased in the aortic root of AS mice. A Oil red-O staining for the aortic root of wild-type mice with ND, wild-type mice with HFD, or ApoE−/− mice with HFD. Scale bar = 500 μm (40 ×). B Immunofluorescence double staining for HOXA1 (red) and α-SMA (green) in each group. DAPI (blue) was used for nucleus staining. Scale bar = 50 μm (400 ×). C Western blot for HOXA1 expression in aortic root of wild-type or HFD-fed ApoE−/− mice. Data are expressed as mean ± SD (6 animals/group). Ordinary one-way ANOVA followed by Tukey's multiple comparison test was used to calculate the P value in panel C ***P 

Application: WB    Species: Mouse    Sample:

Fig. 1 HOXA1 expression is increased in the aortic root of AS mice. A Oil red-O staining for the aortic root of wild-type mice with ND, wild-type mice with HFD, or ApoE−/− mice with HFD. Scale bar = 500 μm (40 ×). B Immunofluorescence double staining for HOXA1 (red) and α-SMA (green) in each group. DAPI (blue) was used for nucleus staining. Scale bar = 50 μm (400 ×). C Western blot for HOXA1 expression in aortic root of wild-type or HFD-fed ApoE−/− mice. Data are expressed as mean ± SD (6 animals/group). Ordinary one-way ANOVA followed by Tukey's multiple comparison test was used to calculate the P value in panel C

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