CKI epsilon Antibody - #DF3188
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3188, RRID:AB_2835411.
折りたたみ／展開
Casein kinase 1 epsilon; Casein kinase I; Casein kinase I epsilon; Casein kinase I isoform epsilon; CKI epsilon; CKI-epsilon; CKIe; CKIepsilon; CSNK 1E; CSNK1E; DBT; Doubletime, Drosophila, homolog of; EC 2.7.11.1; epsilon isoform; HCK IE; KC1E; KC1E_HUMAN; kinase CK1 epsilon; MGC 10398;
免疫原
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
- P49674 KC1E_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELRVGNKYRLGRKIGSGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P49674 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S17 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| K45 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| R321 | Methylation | Uniprot | |
| S323 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| T325 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| T334 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| T337 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| R342 | Methylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| S377 | Phosphorylation | Uniprot | |
| R382 | Methylation | Uniprot | |
| S389 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S390 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| T407 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| S408 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
PTMs - P49674 酵素として
| Substrate | Site | Source |
|---|---|---|
| O14640 (DVL1) | S139 | Uniprot |
| O14640 (DVL1) | S142 | Uniprot |
| O14641 (DVL2) | S143 | Uniprot |
| O14641 (DVL2) | T224 | Uniprot |
| O15055-1 (PER2) | S662 | Uniprot |
| O15055-1 (PER2) | S665 | Uniprot |
| O15055 (PER2) | S668 | Uniprot |
| O15055-1 (PER2) | S671 | Uniprot |
| O15055-1 (PER2) | S674 | Uniprot |
| O60716 (CTNND1) | S268 | Uniprot |
| O75581 (LRP6) | S1420 | Uniprot |
| O75581 (LRP6) | S1430 | Uniprot |
| O75581 (LRP6) | S1431 | Uniprot |
| O95863 (SNAI1) | S104 | Uniprot |
| O95863 (SNAI1) | S107 | Uniprot |
| P12830 (CDH1) | S844 | Uniprot |
| P25054 (APC) | S1279 | Uniprot |
| P25054 (APC) | S1392 | Uniprot |
| P25054-1 (APC) | S1504 | Uniprot |
| P25054-1 (APC) | S1505 | Uniprot |
| P25054-1 (APC) | S1507 | Uniprot |
| P25054-1 (APC) | S1510 | Uniprot |
| P35222 (CTNNB1) | S45 | Uniprot |
| P46937 (YAP1) | S400 | Uniprot |
| P46937 (YAP1) | S403 | Uniprot |
| P49674 (CSNK1E) | T44 | Uniprot |
| P49674 (CSNK1E) | S323 | Uniprot |
| P49674 (CSNK1E) | T325 | Uniprot |
| P49674 (CSNK1E) | T334 | Uniprot |
| P49674 (CSNK1E) | T337 | Uniprot |
| P49674 (CSNK1E) | S368 | Uniprot |
| P49674 (CSNK1E) | S405 | Uniprot |
| P49674 (CSNK1E) | T407 | Uniprot |
| P49674 (CSNK1E) | S408 | Uniprot |
| P55957 (BID) | T59 | Uniprot |
| P55957 (BID) | S64 | Uniprot |
| P56645-1 (PER3) | S622 | Uniprot |
| P56645-1 (PER3) | S625 | Uniprot |
| P56645-1 (PER3) | S628 | Uniprot |
| P56645-1 (PER3) | S633 | Uniprot |
| P56645-1 (PER3) | S634 | Uniprot |
| P56645-1 (PER3) | T635 | Uniprot |
| Q13541 (EIF4EBP1) | T41 | Uniprot |
| Q13541 (EIF4EBP1) | T50 | Uniprot |
| Q8TD43 (TRPM4) | S839 | Uniprot |
| Q9BZL6 (PRKD2) | S244 | Uniprot |
| Q9GZV5 (WWTR1) | S314 | Uniprot |
| Q9Y6Q9 (NCOA3) | S102 | Uniprot |
研究背景
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1 and DVL2. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.
Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.
Cytoplasm. Nucleus.
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
Monomer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins (By similarity). Interacts with PER1. Interacts with ANKRD6 (By similarity). Interacts with DBNDD2. Interacts with LRP5 and LRP6. Interacts with SOCS3. Interacts with SNAI1 (via zinc fingers). Interacts with DDX3X; the interaction greatly improves CSNK1E affinity for ATP and enhances DVL2 phosphorylation (By similarity).
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
研究領域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)
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