製品: ZNF265 Antibody
カタログ: DF3054
タンパク質の説明: Rabbit polyclonal antibody to ZNF265
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 34 KD; 37kD(Calculated).
ユニプロット: O95218
RRID: AB_2835437

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
ZNF265 Antibody detects endogenous levels of total ZNF265.
RRID:
AB_2835437
引用形式: Affinity Biosciences Cat# DF3054, RRID:AB_2835437.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ZRANB 2; DKFZp686J1831; DKFZp686N09117; FLJ41119; OTTHUMP00000011240; OTTHUMP00000011241; Zinc finger protein 265; Zinc finger RAN binding domain containing 2; Zinc finger Ran binding domain containing protein 2; Zinc finger splicing; ZIS; ZIS1; ZIS2; ZNF265;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MSTKNFRVSDGDWICPDKKCGNVNFARRTSCNRCGREKTTEAKMMKAGGTEIGKTLAEKSRGLFSANDWQCKTCSNVNWARRSECNMCNTPKYAKLEERTGYGGGFNERENVEYIEREESDGEYDEFGRKKKKYRGKAVGPASILKEVEDKESEGEEEDEDEDLSKYKLDEDEDEDDADLSKYNLDASEEEDSNKKKSNRRSRSKSRSSHSRSSSRSSSPSSSRSRSRSRSRSSSSSQSRSRSSSRERSRSRGSKSRSSSRSHRGSSSPRKRSYSSSSSSPERNRKRSRSRSSSSGDRKKRRTRSRSPERRHRSSSGSSHSGSRSSSKKK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95218 基板として

Site PTM Type Enzyme
S9 Phosphorylation
T50 Phosphorylation
K54 Acetylation
K54 Ubiquitination
K59 Acetylation
K59 Ubiquitination
R61 Methylation
S65 Phosphorylation
S83 Phosphorylation
T90 Phosphorylation
K92 Acetylation
Y93 Phosphorylation
K95 Sumoylation
K95 Ubiquitination
Y102 Phosphorylation
Y114 Phosphorylation
S120 Phosphorylation
Y124 Phosphorylation
K137 Methylation
S143 Phosphorylation
S153 Phosphorylation
S165 Phosphorylation
K168 Ubiquitination
S181 Phosphorylation
K182 Ubiquitination
Y183 Phosphorylation
S188 Phosphorylation
S193 Phosphorylation
S206 Phosphorylation
S208 Phosphorylation
S213 Phosphorylation
S217 Phosphorylation
S218 Phosphorylation
S221 Phosphorylation
S249 Phosphorylation
S273 Phosphorylation
S275 Phosphorylation
S279 Phosphorylation
S280 Phosphorylation
S288 Phosphorylation
S290 Phosphorylation
T303 Phosphorylation
S305 Phosphorylation
S307 Phosphorylation
S318 Phosphorylation
S323 Phosphorylation
S326 Phosphorylation

PTMs - O95218 酵素として

Substrate Site Source
O95218-2 (ZRANB2) S188 Uniprot

研究背景

機能:

Splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. May interfere with constitutive 5'-splice site selection.

PTMs:

Isoform 2 is phosphorylated on Ser-310 upon DNA damage, probably by ATM or ATR.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with the C-terminal half of SNRNP70, the Arg/Ser-rich domain of AKAP17A as well as with U2AF1 and CLK1.

タンパク質ファミリー:

The RanBP2-type zinc fingers mediate binding to RNA.

Belongs to the ZRANB2 family.

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