GAK Antibody - #AF0317
(3)
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse
予測:
Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%), Dog(82%)
クローナリティ:
Polyclonal
特異性:
GAK Antibody detects endogenous levels of total GAK.
RRID:
AB_2833481
引用形式: Affinity Biosciences Cat# AF0317, RRID:AB_2833481.
引用形式: Affinity Biosciences Cat# AF0317, RRID:AB_2833481.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
Auxilin 2; Cyclin G associated kinase; Cyclin-G-associated kinase; DNAJ26; DNAJC26; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; GAK protein; GAK_HUMAN; MGC99654;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質の説明:
GAK Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily.
タンパク質配列:
- O14976 GAK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGPPPPVGPAGSGYSGGLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESALKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSRFHNRVSECGWAARRAPHLHTLYNICRNMHAWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVRAVVMTPVPLFSKQRSGCRPFCEVYVGDERVASTSQEYDKMRDFKIEDGKAVIPLGVTVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENSSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAGAGSPEAEPTDSDSPPSSSADASRFLHTLDWQEEKEAETGAENASSKESESALMEDRDESEVSDEGGSPISSEGQEPRADPEPPGLAAGLVQQDLVFEVETPAVLPEPVPQEDGVDLLGLHSEVGAGPAVPPQACKAPSSNTDLLSCLLGPPEAASQGPPEDLLSEDPLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSAPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
82
Bovine
82
Sheep
82
Dog
82
Rabbit
82
Chicken
73
Zebrafish
55
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14976 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| Y201 | Phosphorylation | Uniprot | |
| Y204 | Phosphorylation | Uniprot | |
| Y235 | Phosphorylation | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| Y285 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| Y338 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| Y367 | Phosphorylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| K395 | Acetylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| Y412 | Phosphorylation | P12931 (SRC) | Uniprot |
| S421 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| R439 | Methylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| Y615 | Phosphorylation | Uniprot | |
| K617 | Ubiquitination | Uniprot | |
| K682 | Ubiquitination | Uniprot | |
| K685 | Ubiquitination | Uniprot | |
| K748 | Ubiquitination | Uniprot | |
| K751 | Ubiquitination | Uniprot | |
| Y764 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| T776 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| S785 | Phosphorylation | Uniprot | |
| T794 | Phosphorylation | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| T805 | Phosphorylation | Uniprot | |
| S811 | Phosphorylation | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| S817 | Phosphorylation | Uniprot | |
| S826 | Phosphorylation | Uniprot | |
| S829 | Phosphorylation | Uniprot | |
| S834 | Phosphorylation | Uniprot | |
| S837 | Phosphorylation | Uniprot | |
| S838 | Phosphorylation | Uniprot | |
| T908 | Phosphorylation | Uniprot | |
| S939 | Phosphorylation | Uniprot | |
| S1096 | Phosphorylation | Uniprot | |
| S1118 | Phosphorylation | Uniprot | |
| S1130 | Phosphorylation | Uniprot | |
| Y1149 | Phosphorylation | Uniprot | |
| S1151 | Phosphorylation | Uniprot | |
| S1154 | Phosphorylation | Uniprot | |
| S1168 | Phosphorylation | Uniprot | |
| K1172 | Ubiquitination | Uniprot | |
| S1176 | Phosphorylation | Uniprot | |
| S1185 | Phosphorylation | Uniprot | |
| R1192 | Methylation | Uniprot | |
| T1213 | Phosphorylation | Uniprot | |
| K1217 | Ubiquitination | Uniprot | |
| K1219 | Ubiquitination | Uniprot | |
| K1278 | Ubiquitination | Uniprot |
研究背景
機能:
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
細胞の位置付け:
Cytoplasm>Perinuclear region. Golgi apparatus>trans-Golgi network. Cell junction>Focal adhesion.
Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
組織特異性:
Ubiquitous. Highest in testis.
タンパク質ファミリー:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
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