M-CK Antibody - #DF3072
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3072, RRID:AB_2835455.
折りたたみ／展開
CKM; CKMM; Creatine kinase M; Creatine kinase M chain; Creatine kinase M type; Creatine kinase M-type; Creatine kinase muscle; Creatine kinase, muscle type; KCRM_HUMAN; M-CK; MCK; Muscle creatine kinase;
免疫原
- P06732 KCRM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIMTVGCVAGDEESYEVFKELFDPIISDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYVLTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P06732 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y14 | Phosphorylation | Uniprot | |
| Y20 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| K40 | Acetylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| K170 | Acetylation | Uniprot | |
| Y173 | Phosphorylation | Uniprot | |
| Y174 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| R215 | Methylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K247 | Acetylation | Uniprot | |
| K265 | Acetylation | Uniprot | |
| Y279 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K365 | Acetylation | Uniprot | |
| S372 | Phosphorylation | Uniprot |
研究背景
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Cytoplasm.
Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
Belongs to the ATP:guanido phosphotransferase family.
研究領域
· Metabolism > Amino acid metabolism > Arginine and proline metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.