HOXC6 Antibody - #DF3078

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製品: HOXC6 Antibody
カタログ: DF3078
タンパク質の説明: Rabbit polyclonal antibody to HOXC6
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 26 KD; 27kD(Calculated).
ユニプロット: P09630
RRID: AB_2835461

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
HOXC6 Antibody detects endogenous levels of total HOXC6.
RRID:
AB_2835461
引用形式: Affinity Biosciences Cat# DF3078, RRID:AB_2835461.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CP25; HHO.C8; Homeo box 3C; Homeo box C6; Homeo box C8 protein; Homeobox C6; Homeobox protein CP25; Homeobox protein HHO.C8; Homeobox protein Hox-3C; Homeobox protein Hox-C6; HOX3; HOX3C; Hoxc6; HXC6_HUMAN;

免疫原

免疫原:
Uniprot:

P09630(HXC6_HUMAN) >>Visit HPA database.

遺伝子(ID):
HOXC6(3223)
タンパク質配列:
MNSYFTNPSLSCHLAGGQDVLPNVALNSTAYDPVRHFSTYGAAVAQNRIYSTPFYSPQENVVFSSSRGPYDYGSNSFYQEKDMLSNCRQNTLGHNTQTSIAQDFSSEQGRTAPQDQKASIQIYPWMQRMNSHSGVGYGADRRRGRQIYSRYQTLELEKEFHFNRYLTRRRRIEIANALCLTERQIKIWFQNRRMKWKKESNLTSTLSGGGGGATADSLGGKEEKREETEEEKQKE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09630 基板として

Site PTM Type Enzyme
S131 Phosphorylation
Y137 Phosphorylation
K158 Ubiquitination
R164 Methylation
K198 Acetylation
K198 Ubiquitination
K221 Acetylation

研究背景

機能:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the Antp homeobox family.

参考文献

1). Expression Profiles of HOXC6 Predict the Survival of Glioblastoma Patients and Correlate with Cell Cycle. Journal of Oncology, 2022 (PubMed: 35432534)

Application: WB    Species: Human    Sample: LN229 cells

Figure 5 Tumor cell biology experiments of HOXC6. (a) Expression of HOXC6 after transfection of siRNA or overexpression plasmid measured by PCR. (b) CCK-8 assay to evaluate the effect of HOXC6 knockdown or overexpression on cell proliferation in LN229 cells. ∗p < 0.05, ∗∗p < 0.01. (c) CCK-8 assay to evaluate the effect of HOXC6 knockdown or overexpression on cell proliferation in T98G cells. From left to right: NC, OE, and SI. (d) Flow cytometry analysis to evaluate the effects of HOXC6 on cell cycle progression in LN229 cells. (e) Flow cytometry analysis to evaluate the effects of HOXC6 on cell cycle progression in T98G cells. From left to right: NC, OE, and SI. (f) Western blot analysis to evaluate the effects of HOXC6 on expression level of cell cycle related proteins.

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