HARS Antibody - #DF3087
| 製品: | HARS Antibody |
| カタログ: | DF3087 |
| タンパク質の説明: | Rabbit polyclonal antibody to HARS |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 60/52 KD; 57kD(Calculated). |
| ユニプロット: | P12081 |
| RRID: | AB_2835470 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3087, RRID:AB_2835470.
折りたたみ／展開
cytoplasmic; EC 6.1.1.21; FLJ20491; HARS; HisRS; Histidine tRNA ligase, cytoplasmic; histidine translase; Histidine tRNA ligase; Histidine--tRNA ligase; Histidyl tRNA synthetase; Histidyl-tRNA synthetase; HRS; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds; SYHC_HUMAN; USH3B;
免疫原
- P12081 HARS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P12081 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K57 | Methylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| T58 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| K112 | Acetylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| Y138 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| Y363 | Phosphorylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K443 | Acetylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| K499 | Ubiquitination | Uniprot |
研究背景
Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.
Cytoplasm.
Brain, heart, liver and kidney.
Homodimer.
Belongs to the class-II aminoacyl-tRNA synthetase family.
研究領域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
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