MYF5 Antibody - #DF3089

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製品: MYF5 Antibody
カタログ: DF3089
タンパク質の説明: Rabbit polyclonal antibody to MYF5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit
分子量: 30-40kD; 28kD(Calculated).
ユニプロット: P13349
RRID: AB_2835472

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(89%), Horse(100%), Sheep(89%), Rabbit(89%)
クローナリティ:
Polyclonal
特異性:
MYF5 Antibody detects endogenous levels of total MYF5.
RRID:
AB_2835472
引用形式: Affinity Biosciences Cat# DF3089, RRID:AB_2835472.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

bHLHc2; Class C basic helix loop helix protein 2; Class C basic helix-loop-helix protein 2; Myf 5; Myf-5; Myf5; MYF5_HUMAN; Myogenic factor 5;

免疫原

免疫原:
Uniprot:

P13349(MYF5_HUMAN) >>Visit HPA database.

遺伝子(ID):
MYF5(4617)
タンパク質配列:
MDVMDGCQFSPSEYFYDGSCIPSPEGEFGDEFVPRVAAFGAHKAELQGSDEDEHVRAPTGHHQAGHCLMWACKACKRKSTTMDRRKAATMRERRRLKKVNQAFETLKRCTTTNPNQRLPKVEILRNAIRYIESLQELLREQVENYYSLPGQSCSEPTSPTSNCSDGMPECNSPVWSRKSSTFDSIYCPDVSNVYATDKNSLSSLDCLSNIVDRITSSEQPGLPLQDLASLSPVASTDSQPATPGASSSRLIYHVL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
89
Sheep
89
Rabbit
89
Zebrafish
78
Dog
75
Pig
67
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13349 基板として

Site PTM Type Enzyme
S49 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S133 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)

研究背景

機能:

Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Efficient DNA binding requires dimerization with another bHLH protein.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

参考文献

1). SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice. CELL DEATH AND DIFFERENTIATION, 2024 [IF=13.7]
2). N-Acetylcysteine assists muscle development in offspring of mice subjected to maternal heat stress during pregnancy. Journal of the science of food and agriculture, 2024 (PubMed: 38828636) [IF=4.1]
3). Nec-1 alleviated the deleterious effect of CoCl2 on C2C12 myoblast differentiation and fusion via the mTOR pathway. Tissue and Cell, 2022 (PubMed: 36081319) [IF=2.6]

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