TRI18 Antibody - #AF0321
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0321, RRID:AB_2833484.
折りたたみ／展開
BBBG 1; BBBG1; E3 ubiquitin-protein ligase Midline-1; Finger on X and Y mouse homolog of; FXY; GBBB 1; GBBB1; MID 1; Mid1; Midin; Midline 1 (Opitz/BBB syndrome); Midline 1; Midline 1 ring finger; Midline 1 RING finger protein; Midline-1; Midline1; OGS 1; OGS1; OS; OSX; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; RNF 59; RNF59; TRI18; TRI18_HUMAN; TRIM 18; TRIM18; Tripartite motif containing protein 18; Tripartite motif protein TRIM18; Tripartite motif-containing protein 18; XPRF; zinc finger on X and Y, mouse, homolog of; Zinc finger X and Y; ZNFXY;
免疫原
In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
- O15344 TRI18_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O15344 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S66 | Phosphorylation | Uniprot | |
| R68 | Methylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| T110 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| Y378 | Phosphorylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| T505 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| T518 | Phosphorylation | Uniprot | |
| Y528 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot |
研究背景
Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
Phosphorylated on serine and threonine residues.
Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle.
Note: Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
Homodimer or heterodimer with MID2. Interacts with IGBP1. Interacts with TRIM16.
Belongs to the TRIM/RBCC family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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