K6PL Antibody - #DF3107
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3107, RRID:AB_2835484.
折りたたみ／展開
;PFK liver isoenzyme; 6 phosphofructokinase, liver type; K6PL; liver phosphofructokinase; liver type 1 phosphofructokinase; PFK B; PFK liver isozyme; Phosphofructo 1 kinase isozyme B; Phosphofructokinase 1; Phosphofructokinase, liver; Phosphohexokinase;
免疫原
- P17858 PFKAL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVDLEKLRASGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTIIGSARCKAFTTREGRRAAAYNLVQHGITNLCVIGGDGSLTGANIFRSEWGSLLEELVAEGKISETTARTYSHLNIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSSYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVTLSGNQSVRLPLMECVQMTKEVQKAMDDKRFDEATQLRGGSFENNWNIYKLLAHQKPPKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISHGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIHALLVVGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLVLRNEKCHDYYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWLSEKLREVYRKGRVFANAPDSACVIGLKKKAVAFSPVTELKKDTDFEHRMPREQWWLSLRLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDKGF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P17858 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| Y43 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| R301 | Methylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| R310 | Methylation | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| R374 | Methylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| R461 | Methylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| K469 | Acetylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| T471 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S529 | O-Glycosylation | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| K563 | Acetylation | Uniprot | |
| K614 | Ubiquitination | Uniprot | |
| K616 | Ubiquitination | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| Y633 | Phosphorylation | Uniprot | |
| Y634 | Phosphorylation | Uniprot | |
| Y640 | Phosphorylation | Uniprot | |
| R654 | Methylation | Uniprot | |
| R672 | Methylation | Uniprot | |
| Y674 | Phosphorylation | Uniprot | |
| K677 | Ubiquitination | Uniprot | |
| K689 | Acetylation | Uniprot | |
| Y694 | Phosphorylation | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K726 | Acetylation | Uniprot | |
| K726 | Ubiquitination | Uniprot | |
| K727 | Acetylation | Uniprot | |
| S763 | Phosphorylation | Uniprot | |
| T773 | Phosphorylation | Uniprot | |
| S775 | Phosphorylation | Uniprot | |
| K778 | Ubiquitination | Uniprot |
研究背景
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).
GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation.
Cytoplasm.
Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet). The composition of the PFK tetramer differs according to the tissue type it is present in. In muscles, it is composed of 4 PFKM subunits (also called M4). In the liver, the predominant form is a tetramer of PFKL subunits (L4). In erythrocytes, both PFKM and PFKL subunits randomly tetramerize to form M4, L4 and other combinations (ML3, M2L2, M3L). The kinetic and regulatory properties of the tetrameric enzyme are dependent on the subunit composition, hence can vary across tissues (Probable).
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.
研究領域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.
· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.
· Metabolism > Carbohydrate metabolism > Galactose metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
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