Cytokeratin 18 Recombinant Rabbit mAb - #BF3434

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製品: Cytokeratin 18 Recombinant Rabbit mAb
カタログ: BF3434
タンパク質の説明: Rabbit monoclonal antibody to Cytokeratin 18
アプリケーション: WB IHC IF/ICC
反応性: Human, Rat
分子量: 48 kDa(Observed); 48kD(Calculated).
ユニプロット: P05783

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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000, IHC 1:200-1:1000, IF/ICC 1:50
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Monoclonal [ReFirm22881]
特異性:
Cytokeratin 18 Recombinant Rabbit mAb detects endogenous levels of Cytokeratin 18.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; K 18; K18; K1C18_HUMAN; KA18; Keratin 18; Keratin 18, type I; Keratin D; keratin, type I cytoskeletal 18; Keratin-18; Krt18;

免疫原

免疫原:

A synthetic peptide from human Cytokeratin 18

Uniprot:

P05783(K1C18_HUMAN) >>Visit HPA database.

遺伝子(ID):
KRT18(3875)
発現特異性:
P05783 K1C18_HUMAN:

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

タンパク質の説明:
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18,together with its filament partner keratin 8,are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
タンパク質配列:
MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

研究背景

機能:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

PTMs:

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.

Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.

O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

細胞の位置付け:

Cytoplasm>Perinuclear region. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

タンパク質ファミリー:

Belongs to the intermediate filament family.

研究領域

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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