KAP1 Antibody - #DF3150
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3150, RRID:AB_2835527.
折りたたみ／展開
cAMP dependent protein kinase type I beta regulatory subunit; cAMP-dependent protein kinase type I-beta regulatory subunit; KAP1_HUMAN; PKARI beta; PRKAR 1; PRKAR 1B; PRKAR1; Prkar1b; PRKAR1B protein; Protein kinase cAMP dependent regulatory type I beta; RIbeta;
免疫原
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
- P31321 KAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDEEVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAHLDDNERSDIFDAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKLDRPRFERVLGPCSEILKRNIQRYNSFISLTV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P31321 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| T85 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| R342 | Methylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot |
研究背景
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
Cell membrane.
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Interacts with C2orf88/smAKAP; this interaction may target PRKAR1B to the plasma membrane.
Belongs to the cAMP-dependent kinase regulatory chain family.
研究領域
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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