GRK4 Antibody - #DF3152
| 製品: | GRK4 Antibody |
| カタログ: | DF3152 |
| タンパク質の説明: | Rabbit polyclonal antibody to GRK4 |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Rabbit, Chicken |
| 分子量: | 66 KD; 67kD(Calculated). |
| ユニプロット: | P32298 |
| RRID: | AB_2835529 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3152, RRID:AB_2835529.
折りたたみ／展開
G protein coupled receptor kinase 4; G protein-coupled receptor kinase 2 (Drosophila)-like; G protein-coupled receptor kinase 4; G protein-coupled receptor kinase GRK4; G proteinm coupled receptor kinase GRK4; GPRK2L; GPRK4; GRK4; GRK4_HUMAN; GRK4a; ITI1;
免疫原
Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium.
- P32298 GRK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELENIVANSLLLKARQGGYGKKSGRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRRLFRQFCDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGIYLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALPLDLDKNIHTPVSRPNRGFFYRLFRRGGCLTMVPSEKEVEPKQC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P32298 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S10 | Phosphorylation | P32298 (GRK4) | Uniprot |
| K14 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | P32298 (GRK4) | Uniprot |
| K184 | Ubiquitination | Uniprot | |
| K195 | Acetylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | P32298 (GRK4) | Uniprot |
| S249 | Phosphorylation | P32298 (GRK4) | Uniprot |
| T256 | Phosphorylation | P32298 (GRK4) | Uniprot |
| Y409 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | P32298 (GRK4) | Uniprot |
| T426 | Phosphorylation | P32298 (GRK4) | Uniprot |
| S430 | Phosphorylation | P32298 (GRK4) | Uniprot |
| S485 | Phosphorylation | P32298 (GRK4) | Uniprot |
| K527 | Acetylation | Uniprot |
PTMs - P32298 酵素として
| Substrate | Site | Source |
|---|---|---|
| P30411-1 (BDKRB2) | S366 | Uniprot |
| P30411-1 (BDKRB2) | S373 | Uniprot |
| P30411 (BDKRB2) | S375 | Uniprot |
| P32298 (GRK4) | S10 | Uniprot |
| P32298 (GRK4) | S139 | Uniprot |
| P32298 (GRK4) | S244 | Uniprot |
| P32298 (GRK4) | S249 | Uniprot |
| P32298 (GRK4) | T256 | Uniprot |
| P32298 (GRK4) | S419 | Uniprot |
| P32298 (GRK4) | T426 | Uniprot |
| P32298 (GRK4) | S430 | Uniprot |
| P32298 (GRK4) | S485 | Uniprot |
研究背景
Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.
Palmitoylated.
Cytoplasm. Cytoplasm>Cell cortex.
Note: Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where colocalization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 colocalize in lipid rafts of renal proximal tubule cells.
Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium.
Interacts with DRD3.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
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