TF2H1 Antibody - #DF3155
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3155, RRID:AB_2835532.
折りたたみ／展開
Basic transcription factor 2 62 kDa subunit; Basic transcription factor 62 kDa subunit; BTF 2; BTF2; BTF2 p62; General transcription factor IIH polypeptide 1; General transcription factor IIH subunit 1; General transcription factor IIH, polypeptide 1, 62kDa; gtf2h1; TF2H1; TF2H1_HUMAN; TFB1; TFIIH; TFIIH basal transcription factor complex p62 subunit;
免疫原
- P32780 TF2H1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEGYGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVKRAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P32780 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y25 | Phosphorylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| K240 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| Y360 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| T415 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot |
研究背景
Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Nucleus.
Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60.
Belongs to the TFB1 family.
研究領域
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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