GRK5 Antibody - #DF3159
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3159, RRID:AB_2835536.
折りたたみ／展開
FLJ39780; FP2025; G protein coupled receptor kinase 5; G protein coupled receptor kinase GRK5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5; GRK-pan; GRK5; GRK5_HUMAN; Pan-GRK;
免疫原
Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
- P34947 GRK5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRLLFRQFCETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKELFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P34947 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| K24 | Acetylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K29 | Acetylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | P09619 (PDGFRB) | Uniprot |
| Y109 | Phosphorylation | P09619 (PDGFRB) | Uniprot |
| K113 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K226 | Acetylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| Y251 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y253 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y309 | Phosphorylation | P09619 (PDGFRB) | Uniprot |
| Y368 | Phosphorylation | P09619 (PDGFRB) | Uniprot |
| Y408 | Phosphorylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | P34947 (GRK5) | Uniprot |
| T485 | Phosphorylation | P34947 (GRK5) | Uniprot |
| K487 | Ubiquitination | Uniprot | |
| K548 | Methylation | Uniprot | |
| S586 | Phosphorylation | Uniprot |
PTMs - P34947 酵素として
| Substrate | Site | Source |
|---|---|---|
| O75581 (LRP6) | S1448 | Uniprot |
| O75581 (LRP6) | S1450 | Uniprot |
| O75581 (LRP6) | S1490 | Uniprot |
| O75581 (LRP6) | S1508 | Uniprot |
| O75581 (LRP6) | T1572 | Uniprot |
| O75581 (LRP6) | S1590 | Uniprot |
| O75581 (LRP6) | S1607 | Uniprot |
| O76070 (SNCG) | S124 | Uniprot |
| P04637 (TP53) | T55 | Uniprot |
| P06748 (NPM1) | S4 | Uniprot |
| P07550 (ADRB2) | S355 | Uniprot |
| P07550 (ADRB2) | S356 | Uniprot |
| P07550 (ADRB2) | T384 | Uniprot |
| P07550 (ADRB2) | T393 | Uniprot |
| P07550 (ADRB2) | S396 | Uniprot |
| P07550 (ADRB2) | S401 | Uniprot |
| P07550 (ADRB2) | S407 | Uniprot |
| P07550 (ADRB2) | S411 | Uniprot |
| P25963 (NFKBIA) | S32 | Uniprot |
| P26038 (MSN) | T66 | Uniprot |
| P30411 (BDKRB2) | S366 | Uniprot |
| P30411 (BDKRB2) | S373 | Uniprot |
| P30411-1 (BDKRB2) | S375 | Uniprot |
| P34947 (GRK5) | S484 | Uniprot |
| P34947 (GRK5) | T485 | Uniprot |
| P37840-1 (SNCA) | S129 | Uniprot |
| P49407 (ARRB1) | S412 | Uniprot |
| P50502 (ST13) | S346 | Uniprot |
| P61073 (CXCR4) | S339 | Uniprot |
| Q16143 (SNCB) | S118 | Uniprot |
研究背景
Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity.
Cytoplasm. Nucleus. Cell membrane>Peripheral membrane protein.
Note: Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion.
Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
Interacts with ST13 (via the C-terminus 303-319 AA). Interacts with TP53/p53. Interacts with HTR4 (via C-terminus 330-346 AA); this interaction is promoted by 5-HT (serotonin). Interacts with HDAC5 (By similarity).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
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