Actinin alpha 2/3 Antibody - #DF3163

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製品: Actinin alpha 2/3 Antibody
カタログ: DF3163
タンパク質の説明: Rabbit polyclonal antibody to Actinin alpha 2/3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 103 KD; 104kD,103kD(Calculated).
ユニプロット: P35609 | Q08043
RRID: AB_2835540

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
Actinin alpha 2/3 Antibody detects endogenous levels of total Actinin alpha 2/3.
RRID:
AB_2835540
引用形式: Affinity Biosciences Cat# DF3163, RRID:AB_2835540.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Actin binding protein; Actinin alpha 2; ACTN 2; ACTN2; ACTN2_HUMAN; Alpha actinin 2; Alpha actinin skeletal muscle; Alpha actinin skeletal muscle isoform 2; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; CMD1AA; F actin cross linking protein; F-actin cross-linking protein; Actinin alpha 3; actinin, alpha; ACTN 3; ACTN3; ACTN3_HUMAN; Alpha actinin skeletal muscle; Alpha actinin skeletal muscle isoform 3; Alpha-Actinin 3; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-3; F actin cross linking protein; F-actin cross-linking protein; MGC117002; MGC117005; OTTHUMP00000219049; OTTHUMP00000219054;

免疫原

免疫原:
Uniprot:

P35609(ACTN2_HUMAN) >>Visit HPA database.

Q08043(ACTN3_HUMAN) >>Visit HPA database.

遺伝子(ID):
ACTN2(88) | ACTN3(89)
発現特異性:
P35609 ACTN2_HUMAN:

Expressed in both skeletal and cardiac muscle.

Q08043 ACTN3_HUMAN:

Expressed only in a subset of type 2 skeletal muscle fibers.

タンパク質配列:
MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERMEKLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL

MMMVMQPEGLGAGEGRFAGGGGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTVPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRIQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFRQKASLHEAWTRGKEEMLSQRDYDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDRLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQTLQEELARQQVNERLRRQFAAQANAIGPWIQAKVEEVGRLAAGLAGSLEEQMAGLRQQEQNIINYKTNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKQNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTTEQVVASFKILAGDKNYITPEELRRELPAKQAEYCIRRMVPYKGSGAPAGALDYVAFSSALYGESDL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35609/Q08043 基板として

Site PTM Type Enzyme
K38 Ubiquitination
K42 Ubiquitination
K54 Ubiquitination
T57 Phosphorylation
R83 Methylation
K86 Methylation
Y106 Phosphorylation
S116 Phosphorylation
T130 Phosphorylation
S147 Phosphorylation
T151 Phosphorylation
S152 Phosphorylation
K181 Acetylation
Y200 Phosphorylation
S201 Phosphorylation
T237 Phosphorylation
S250 Phosphorylation
Y253 Phosphorylation
R268 Methylation
S291 Phosphorylation
Y326 Phosphorylation
K338 Ubiquitination
T347 Phosphorylation
T350 Phosphorylation
S355 Phosphorylation
Y385 Phosphorylation
S431 Phosphorylation
S433 Phosphorylation
T435 Phosphorylation
K443 Acetylation
K443 Ubiquitination
S449 Phosphorylation
K499 Acetylation
K523 Acetylation
S588 Phosphorylation
Y589 Phosphorylation
S594 Phosphorylation
S595 Phosphorylation
S596 Phosphorylation
Y599 Phosphorylation
Y681 Phosphorylation
Y715 Phosphorylation
S840 Phosphorylation
Y844 Phosphorylation
Y861 Phosphorylation
S892 Phosphorylation
Site PTM Type Enzyme
M1 Acetylation
K45 Ubiquitination
K49 Ubiquitination
K61 Ubiquitination
T64 Phosphorylation
R90 Methylation
R93 Methylation
S116 Phosphorylation
K117 Ubiquitination
T137 Phosphorylation
S154 Phosphorylation
T158 Phosphorylation
S159 Phosphorylation
K209 Sumoylation
K212 Sumoylation
Y229 Phosphorylation
K250 Acetylation
S257 Phosphorylation
Y260 Phosphorylation
R275 Methylation
K278 Acetylation
S298 Phosphorylation
S319 Phosphorylation
S321 Phosphorylation
Y333 Phosphorylation
T354 Phosphorylation
T357 Phosphorylation
S376 Phosphorylation
S418 Phosphorylation
Y436 Phosphorylation
S438 Phosphorylation
S456 Phosphorylation
K506 Acetylation
S737 Phosphorylation
Y851 Phosphorylation
Y876 Phosphorylation

研究背景

機能:

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

PTMs:

Ubiquitinated by FBXL22, leading to proteasomal degradation.

細胞の位置付け:

Cytoplasm>Myofibril>Sarcomere>Z line.
Note: Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in both skeletal and cardiac muscle.

サブユニット構造:

Homodimer; antiparallel. Also forms heterodimers with ACTN3. Interacts with ADAM12, MYOZ1, MYOZ2 and MYOZ3. Interacts via its C-terminal region with the LDB3 PDZ domain. Interacts with XIRP2. Interacts with DST isoform 1 (via N-terminus). Interacts with PARVB. Interacts with SYNPO2.

タンパク質ファミリー:

Belongs to the alpha-actinin family.

機能:

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

組織特異性:

Expressed only in a subset of type 2 skeletal muscle fibers.

サブユニット構造:

Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1.

タンパク質ファミリー:

Belongs to the alpha-actinin family.

研究領域

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

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