ACHE Recombinant Rabbit mAb - #BF3660

WB 1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Human,Mouse,Rat

Monoclonal [ReFirm23107]

ACHE Recombinant Rabbit mAb detects endogenous levels of ACHE.

Unconjugated.

Affinity-chromatography.

Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

P22303(ACES_HUMAN) >>Visit HPA database.

P22303 ACES_HUMAN:

Isoform H is highly expressed in erythrocytes.

Acetylcholinesterase hydrolyzes the neurotransmitter,acetylcholine at neuromuscular junctions and brain cholinergic synapses,and thus terminates signal transmission. It is also found on the red blood cell membranes,where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties,but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene,and the structural diversity in the gene products arises from alternative mRNA splicing,and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain,muscle and other tissues is the hydrophilic species,which forms disulfide-linked oligomers with collagenous,or lipid-containing structural subunits. The other,alternatively spliced form,expressed primarily in the erythroid tissues,differs at the C-terminal end,and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo,and thus,may be of aid in understanding the effects of transfusion

•    P22303 ACES_HUMAN:
• Protein BLAST With
• NCBI/
• ExPASy/
• Uniprot

MRPPQCLLHTPSLASPLLLLLLWLLGGGVGAEGREDAELLVTVRGGRLRGIRLKTPGGPVSAFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQSVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLVWIYGGGFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREAPGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESAGAASVGMHLLSPPSRGLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCPPGGTGGNDTELVACLRTRPAQVLVNHEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAEFLAGVRVGVPQVSDLAAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIFGIPLDPSRNYTAEEKIFAQRLMRYWANFARTGDPNEPRDPKAPQWPPYTAGAQQYVSLDLRPLEVRRGLRAQACAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQDRCSDL