TOP3A Antibody - #DF3265
| 製品: | TOP3A Antibody |
| カタログ: | DF3265 |
| タンパク質の説明: | Rabbit polyclonal antibody to TOP3A |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Rabbit, Dog, Xenopus |
| 分子量: | 115 KD; 112kD(Calculated). |
| ユニプロット: | Q13472 |
| RRID: | AB_2835563 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3265, RRID:AB_2835563.
折りたたみ／展開
DNA topoisomerase 3 alpha; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; TOP 3; TOP3; TOP3A; TOP3A_HUMAN; topo III-alpha; topoisomerase (DNA) III alpha; topoisomerase (DNA) III; topoisomerase III alpha; ZGRF7; zinc finger, GRF-type containing 7;
免疫原
- Q13472 TOP3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13472 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S22 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| T554 | Phosphorylation | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| K602 | Ubiquitination | Uniprot | |
| K668 | Acetylation | Uniprot | |
| T669 | Phosphorylation | Uniprot | |
| K670 | Acetylation | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| S760 | Phosphorylation | Uniprot | |
| S771 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| S964 | Phosphorylation | Uniprot | |
| S970 | Phosphorylation | Uniprot | |
| S971 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Uniprot | |
| S976 | Phosphorylation | Uniprot | |
| T977 | Phosphorylation | Uniprot | |
| S985 | Phosphorylation | Uniprot |
研究背景
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2.
Mitochondrion matrix.
High expression is found in testis, heart, skeletal muscle and pancreas.
Binds ssDNA. Interacts (via N-terminal region) with BLM; the interaction is direct. Directly interacts with RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM.
Belongs to the type IA topoisomerase family.
研究領域
· Genetic Information Processing > Replication and repair > Homologous recombination.
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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