EPHB4 Antibody - #DF3215
| 製品: | EPHB4 Antibody |
| カタログ: | DF3215 |
| タンパク質の説明: | Rabbit polyclonal antibody to EPHB4 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 108~130kD; 108kD(Calculated). |
| ユニプロット: | P54760 |
| RRID: | AB_2835595 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3215, RRID:AB_2835595.
折りたたみ／展開
Ephb4; EPHB4_HUMAN; Ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma Transmembrane Kinase; HTK; MYK 1; MYK1; TYRO 11; TYRO11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11;
免疫原
Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
- P54760 EPHB4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P54760 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T34 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| T544 | Phosphorylation | Uniprot | |
| Y574 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| Y581 | Phosphorylation | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| Y590 | Phosphorylation | Uniprot | |
| T595 | Phosphorylation | Uniprot | |
| Y596 | Phosphorylation | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| Y614 | Phosphorylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| T713 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| T773 | Phosphorylation | Uniprot | |
| Y774 | Phosphorylation | Uniprot | |
| T775 | Phosphorylation | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S777 | Phosphorylation | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| K885 | Ubiquitination | Uniprot | |
| Y906 | Phosphorylation | Uniprot | |
| S911 | Phosphorylation | Uniprot | |
| Y924 | Phosphorylation | Uniprot | |
| S943 | Phosphorylation | Uniprot | |
| T953 | Phosphorylation | Uniprot | |
| K960 | Ubiquitination | Uniprot | |
| S964 | Phosphorylation | Uniprot | |
| K973 | Ubiquitination | Uniprot | |
| T976 | Phosphorylation | Uniprot | |
| Y987 | Phosphorylation | Uniprot |
PTMs - P54760 酵素として
研究背景
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
Phosphorylated; autophosphorylation is stimulated by EFNB2.
Cell membrane>Single-pass type I membrane protein.
Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts with RASA1; the interaction depends on EPHB4 tyrosine-phosphorylation.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
研究領域
· Organismal Systems > Development > Axon guidance. (View pathway)
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