DLX5 Antibody - #DF3220

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製品: DLX5 Antibody
カタログ: DF3220
タンパク質の説明: Rabbit polyclonal antibody to DLX5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 32 KD; 32kD(Calculated).
ユニプロット: P56178
RRID: AB_2835600

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
DLX5 Antibody detects endogenous levels of total DLX5.
RRID:
AB_2835600
引用形式: Affinity Biosciences Cat# DF3220, RRID:AB_2835600.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Distal less homeo box 5; Distal less homeobox 5; Dlx 5; Homeo box protein DLX 5; Homeobox protein DLX 5; OTTHUMP00000205292;

免疫原

免疫原:
Uniprot:

P56178(DLX5_HUMAN) >>Visit HPA database.

遺伝子(ID):
DLX5(1749)
タンパク質配列:
MTGVFDRRVPSIRSGDFQAPFQTSAAMHHPSQESPTLPESSATDSDYYSPTGGAPHGYCSPTSASYGKALNPYQYQYHGVNGSAGSYPAKAYADYSYASSYHQYGGAYNRVPSATNQPEKEVTEPEVRMVNGKPKKVRKPRTIYSSFQLAALQRRFQKTQYLALPERAELAASLGLTQTQVKIWFQNKRSKIKKIMKNGEMPPEHSPSSSDPMACNSPQSPAVWEPQGSSRSLSHHPHAHPPTSNQSPASSYLENSASWYTSAASSINSHLPPPGSLQHPLALASGTLY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56178 基板として

Site PTM Type Enzyme
S34 Phosphorylation Q16539 (MAPK14)
S60 Phosphorylation
Y66 Phosphorylation
K158 Ubiquitination
S217 Phosphorylation Q16539 (MAPK14)

研究背景

機能:

Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.

PTMs:

Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14 enhances its transcriptional activity. Phosphorylation by CaMK2 increases its protein stability (By similarity).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with XRCC6 (Ku70).

タンパク質ファミリー:

Belongs to the distal-less homeobox family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

参考文献

1). Long non-coding RNA RP11-197K6.1 as ceRNA promotes colorectal cancer progression via miR-135a-5p/DLX5 axis. Journal of translational medicine, 2024 (PubMed: 38760791) [IF=7.4]

Application: WB    Species: Human    Sample: HCT116 and SW480 cells

Fig. 3 DLX5 expression analysis and its association with CRC progression. A: Comparison of DLX5 expression levels between CRC tissues and normal tissues based on the TCGA-COAD dataset. B: Kaplan-Meier survival curves for comparison of survival rates between CRC patients with high vs. low DLX5 expression. C: RT-qPCR analysis of DLX5 expression in matched fresh CRC tissues and adjacent normal tissues collected from patients during surgery. D: Comparison of DLX5 expression levels across various CRC cell lines (HCT116, HT-29, LOVO, and SW480) and the control cell line NCM460. E: Gene set enrichment analysis indicated significant enrichment of hypertrophic cardiomyopathy-related genes, the extracellular matrix-receptor interaction pathway, and the TGF-β signaling pathway in CRC patients with high DLX5 expression, thus suggesting the association of these pathways with DLX5 upregulation. F: Pearson’s correlation analysis showed a positive correlation between the expression levels of lncRNA RP11-197K6.1 and DLX5 in CRC tissues. G: Western blotting assays showed a decrease in DLX5 expression following lncRNA RP11-197K6.1 knockdown in HCT116 and SW480 cells (***P 
2). SHFM1 deficiency suppresses esophageal squamous cell carcinomas progression via modulating NF‑κB signaling and enhancing nature killer cell‑mediated tumor surveillance. Experimental and Therapeutic Medicine, 2023 (PubMed: 37090067) [IF=2.7]

Application: IHC    Species: Human    Sample: ESCC tissues

Figure 3 SHFM1 expression based on online database and clinic samples. (A) Expression profile of SHFM1 in patients with ESCC based on different clinical features. (B) The expression of SHFM1 in ESCC tissues was detected by IHC staining. Images were captured at x400 magnification. (C) Expression of SHFM1 in matched 17 ESCC tissues (T) and the non-tumor tissues (N) was explored by western blotting. Relative expression was analyzed and β-actin was used as a normalization control. Data are presented as the mean ± standard deviation. ns, not significant; **P

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