SPOP Recombinant Rabbit mAb - #BF4243

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製品: SPOP Recombinant Rabbit mAb
カタログ: BF4243
タンパク質の説明: Rabbit monoclonal antibody to SPOP
アプリケーション: WB
反応性: Human
分子量: 42 kDa(Observed); 42kD(Calculated).
ユニプロット: O43791

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製品説明

ソース:
Rabbit IgG
アプリケーション:
WB 1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [ReFirm23693]
特異性:
SPOP Recombinant Rabbit mAb detects endogenous levels of SPOP.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BTBD32; HIB homolog 1; Roadkill homolog 1; Speckle type POZ protein; Speckle-type POZ protein; SPOP; SPOP_HUMAN; TEF 2; TEF2;

免疫原

免疫原:

A synthetic peptide from human SPOP

Uniprot:

O43791(SPOP_HUMAN) >>Visit HPA database.

遺伝子(ID):
SPOP(8405)
発現特異性:
O43791 SPOP_HUMAN:

Widely expressed.

タンパク質の説明:
This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX),which interacts with histone deacetylase,core histones,and other histone-associated proteins. In mouse,the encoded protein binds to the putative leucine zipper domain of macroH2A1.2,a variant H2A histone that is enriched on inactivated X chromosomes. The BTB,POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
タンパク質配列:
MSRVPSPPPPAEMSSGPVAESWCYTQIKVVKFSYMWTINNFSFCREEMGEVIKSSTFSSGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCPKSEVRAKFKFSILNAKGEETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQNTMNMVKVPECRLADELGGLWENSRFTDCCLCVAGQEFQAHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVAEAYRSLASAQCPFLGPPRKRLKQS

研究背景

機能:

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.

細胞の位置付け:

Nucleus. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed.

タンパク質ファミリー:

The BTB (POZ) domain mediates dimerization and interaction with CUL3.

The MATH domain mediates interaction with protein-ubiquitin ligase substrates, such as MACROH2A1 and BMI1.

Belongs to the Tdpoz family.

研究領域

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

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