SFRS3 Antibody - #DF3230
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| 製品: | SFRS3 Antibody |
| カタログ: | DF3230 |
| タンパク質の説明: | Rabbit polyclonal antibody to SFRS3 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 25 KD; 19kD(Calculated). |
| ユニプロット: | P84103 |
| RRID: | AB_2835610 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3230, RRID:AB_2835610.
折りたたみ／展開
arginine/serine-rich 3; Pre mRNA splicing factor SRP20; Pre-mRNA-splicing factor SRP20; Serine/arginine-rich splicing factor 3; Splicing factor; Splicing factor arginine/serine rich 20 kD; Splicing factor arginine/serine rich 3; SRp20; SRSF3; SRSF3_HUMAN; X16;
免疫原
- P84103 SRSF3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSRNRGPPPSWGRRPRDDYRRRSPPPRRRSPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNERK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P84103 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| C6 | S-Nitrosylation | Uniprot | |
| C10 | S-Nitrosylation | Uniprot | |
| K11 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| R28 | Methylation | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| Y33 | Phosphorylation | Uniprot | |
| R37 | Methylation | Uniprot | |
| R57 | Methylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K85 | Acetylation | Uniprot | |
| K85 | Sumoylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| R90 | Methylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| R98 | Methylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot |
研究背景
Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. RNA-binding is semi-sequence specific.
Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.
Nucleus. Nucleus speckle. Cytoplasm.
Note: Recruited to nuclear speckles following interaction with YTHDC1.
Interacts with CPSF6. Interacts with RBMY1A1. Interacts with SREK1/SFRS12. Interacts with NXF1. Interacts with YTHDC1, leading to recruitment to RNA elements adjacent to m6A sites.
Belongs to the splicing factor SR family.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
参考文献
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