MtSSB Antibody - #DF3240
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3240, RRID:AB_2835620.
折りたたみ／展開
mitochondrial; Mt SSB; Mt-SSB; MtSSB; PWP1 interacting protein 17; PWP1-interacting protein 17; SENSOR OF SINGLE-STRANDED DNA COMPLEX; Single stranded DNA binding protein 1; Single stranded DNA binding protein; Single stranded DNA binding protein mitochondrial; Single-stranded DNA-binding protein; SOSS B1; SOSS COMPLEX, SUBUNIT B1; SSBP 1; SSBP; SSBP_HUMAN; SSBP1;
免疫原
- Q04837 SSBP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFRRPVLQVLRQFVRHESETTTSLVLERSLNRVHLLGRVGQDPVLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWHRISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q04837 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K51 | Acetylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S70 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| Y99 | Phosphorylation | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| K103 | Acetylation | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| K113 | Acetylation | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot |
研究背景
Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.
Mitochondrion. Mitochondrion matrix>Mitochondrion nucleoid.
Homotetramer. Interacts with MPG/AAG, through inhibition of its glycosylase activity it potentially prevents formation of DNA breaks in ssDNA, ensuring that base removal primarily occurs in dsDNA. Interacts with POLDIP2. Interacts with PRIMPOL.
研究領域
· Genetic Information Processing > Replication and repair > DNA replication.
· Genetic Information Processing > Replication and repair > Mismatch repair.
· Genetic Information Processing > Replication and repair > Homologous recombination.
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