製品: ELAVL2 Antibody
カタログ: DF3251
タンパク質の説明: Rabbit polyclonal antibody to ELAVL2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 38 KD; 40kD(Calculated).
ユニプロット: Q12926
RRID: AB_2835631

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
ELAVL2 Antibody detects endogenous levels of total ELAVL2.
RRID:
AB_2835631
引用形式: Affinity Biosciences Cat# DF3251, RRID:AB_2835631.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ELAV like neuron specific RNA binding protein 2; ELAV like Neuronal Protein 1; ELAV like Protein 2; ELAV-like neuronal protein 1; ELAV-like protein 2; ELAV2_HUMAN; ELAVL2; HEL-N1; HELN1; HU Antigen B; Hu-antigen B; HuB; Nervous System Specific RNA Binding Protein MelN1; Nervous system-specific RNA-binding protein Hel-N1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q12926 ELAV2_HUMAN:

Brain; neural-specific.

タンパク質配列:
METQLSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKTNQAILSQLYQSPNRRYPGPLAQQAQRFRLDNLLNMAYGVKRFSPMTIDGMTSLAGINIPGHPGTGWCIFVYNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12926 基板として

Site PTM Type Enzyme
T51 Phosphorylation
K74 Ubiquitination
K95 Ubiquitination
T99 Phosphorylation
K111 Ubiquitination
Y114 Phosphorylation
S118 Phosphorylation
S119 Phosphorylation
T162 Phosphorylation
S165 Phosphorylation
K209 Ubiquitination
S221 Phosphorylation
K315 Ubiquitination

研究背景

機能:

RNA-binding protein that binds to the 3' untranslated region (3'UTR) of target mRNAs (By similarity). Seems to recognize a GAAA motif (By similarity). Can bind to its own 3'UTR, the FOS 3'UTR and the ID 3'UTR (By similarity).

組織特異性:

Brain; neural-specific.

サブユニット構造:

Interacts with IGF2BP1. Interacts with MAP1B light chain LC1 (By similarity).

タンパク質ファミリー:

Belongs to the RRM elav family.

参考文献

1). Dihydroartemisinin Attenuates Hypoxia-Induced Pulmonary Hypertension Through the ELAVL2/miR-503/PI3K/AKT Axis. Journal of cardiovascular pharmacology, 2022 (PubMed: 35512032) [IF=2.6]

Application: WB    Species: Rat    Sample: PASMCs

FIGURE 6. ELAVL2 regulated PASMCs migration and proliferation negatively. (A), (B), Immunoblotting analysis of ELAVL2 expression in PASMCs transfected with an ELAVL2-overexpressing plasmid or a control plasmid (n =3). (C), (D), Cell migration was determined by transwell assays at 24 hours (n = 3) (×100). (E), (F), EdU incorporation experiments (n = 3) were used to evaluate cell viability (×200). (G), (H), Immunoblotting analysis of ELAVL2 and PCNA expression in PASMCs of each treatment group (n = 3). OE means overexpression of ELAVL2.

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