TNAP1 Antibody - #DF3271
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3271, RRID:AB_2835639.
折りたたみ／展開
alpha-induced protein 1; BACD2_HUMAN; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BTB/POZ domain-containing protein TNFAIP1; endothelial; hBACURD2; Protein B12; tnfaip1; Tumor necrosis factor alpha induced protein 1 (endothelial); Tumor necrosis factor alpha induced protein 1; Tumor necrosis factor;
免疫原
- Q13829 BACD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGDTCLCPASGAKPKLSGFKGGGLGNKYVQLNVGGSLYYTTVRALTRHDTMLKAMFSGRMEVLTDKEGWILIDRCGKHFGTILNYLRDDTITLPQNRQEIKELMAEAKYYLIQGLVNMCQSALQDKKDSYQPVCNIPIITSLKEEERLIESSTKPVVKLLYNRSNNKYSYTSNSDDHLLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKLAEVCCTSIVYATEKKQTKVEFPEARIYEETLNVLLYETPRVPDNSLLEATSRSRSQASPSEDEETFELRDRVRRIHVKRYSTYDDRQLGHQSTHRD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13829 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| Y162 | Phosphorylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| T237 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| Y256 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S280 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T285 | Phosphorylation | Uniprot |
研究背景
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
Cytoplasm. Nucleus. Endosome.
Note: Colocalizes with RHOB in endosomes.
Component of the BCR(TNFAIP1) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with PCNA. Interacts with CSNK2B.
Belongs to the BACURD family.
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