MMP16 Antibody - #AF0214
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0214, RRID:AB_2833284.
折りたたみ／展開
C8orf57; DKFZp761D112; Matrix metalloproteinase 16; Matrix metalloproteinase-16; Membrane type 3 matrix metalloproteinase; Membrane type matrix metalloproteinase 3; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP 16; MMP-16; MMP-X2; MMP16; MMP16_HUMAN; MT-MMP 3; MT-MMP2; MT-MMP3; MT3-MMP; MT3MMP; MTMMP2; MTMMP3; Putative transmembrane protein C8orf57;
免疫原
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
- P51512 MMP16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MILLTFSTGRRLDFVHHSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRKAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGSGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITVWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRVKEGHSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51512 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T5 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| Y134 | Phosphorylation | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| T382 | Phosphorylation | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| Y521 | Phosphorylation | Uniprot |
研究背景
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
The precursor is cleaved by a furin endopeptidase.
Cell membrane>Single-pass type I membrane protein>Extracellular side.
Note: Localized at the cell surface of melanoma cells.
Secreted>Extracellular space>Extracellular matrix. Cell surface.
Note: Localized at the cell surface of melanoma cells.
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Belongs to the peptidase M10A family.
研究領域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
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