CDH11 Antibody - #DF3523

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製品: CDH11 Antibody
カタログ: DF3523
タンパク質の説明: Rabbit polyclonal antibody to CDH11
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 80,100kD; 88kD(Calculated).
ユニプロット: P55287
RRID: AB_2835743

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
クローナリティ:
Polyclonal
特異性:
CDH11 Antibody detects endogenous levels of total CDH11.
RRID:
AB_2835743
引用形式: Affinity Biosciences Cat# DF3523, RRID:AB_2835743.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CAD11; CAD11_HUMAN; Cadherin 11; Cadherin 11 type 2; Cadherin 11 type 2 OB cadherin (osteoblast); Cadherin-11; Cdh11; CDHOB; OB; OB-cadherin; OBcadherin; OSF-4; OSF4; Osteoblast cadherin;

免疫原

免疫原:
Uniprot:

P55287(CAD11_HUMAN) >>Visit HPA database.

遺伝子(ID):
CDH11(1009)
発現特異性:
P55287 CAD11_HUMAN:

Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts.

タンパク質配列:
MKENYCLQAALVCLGMLCHSHAFAPERRGHLRPSFHGHHEKGKEGQVLQRSKRGWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPEFLHETYHANVPERSNVGTSVIQVTASDADDPTYGNSAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREAKEEYHVVIQAKDMGGHMGGLSGTTKVTITLTDVNDNPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIGENGLVTYNIVDGDGMESFEITTDYETQEGVIKLKKPVDFETKRAYSLKVEAANVHIDPKFISNGPFKDTVTVKISVEDADEPPMFLAPSYIHEVQENAAAGTVVGRVHAKDPDAANSPIRYSIDRHTDLDRFFTINPEDGFIKTTKPLDREETAWLNITVFAAEIHNRHQEAKVPVAIRVLDVNDNAPKFAAPYEGFICESDQTKPLSNQPIVTISADDKDDTANGPRFIFSLPPEIIHNPNFTVRDNRDNTAGVYARRGGFSRQKQDLYLLPIVISDGGIPPMSSTNTLTIKVCGCDVNGALLSCNAEAYILNAGLSTGALIAILACIVILLVIVVLFVTLRRQKKEPLIVFEEEDVRENIITYDDEGGGEEDTEAFDIATLQNPDGINGFIPRKDIKPEYQYMPRPGLRPAPNSVDVDDFINTRIQEADNDPTAPPYDSIQIYGYEGRGSVAGSLSSLESATTDSDLDYDYLQNWGPRFKKLADLYGSKDTFDDDS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Zebrafish
78
Xenopus
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55287 基板として

Site PTM Type Enzyme
K43 Acetylation
S93 Phosphorylation
T99 Phosphorylation
T219 Phosphorylation
K365 Ubiquitination
Y700 Phosphorylation
Y702 Phosphorylation
S714 Phosphorylation
T723 Phosphorylation
S788 Phosphorylation
T791 Phosphorylation

研究背景

機能:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts.

サブユニット構造:

Interacts with PCDH8.

タンパク質ファミリー:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

参考文献

1). MiRNA-451a inhibits airway remodeling by targeting Cadherin 11 in an allergic asthma model of neonatal mice. International Immunopharmacology, 2020 (PubMed: 32234673) [IF=4.8]

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Fig. 1. Opposite expression of miRNA-451a and CDH11 in the lung of asthma mouse pups. (A) New born mice were intraperitoneally injected with 10 μg ovalbumin (OVA) from postnatal day 5 (P5) to P10, and followed by 10-min daily challenge with 3% aerosolized OVA from P18 to P20. Twenty-four hours post the last OVA challenge (P21), the bronchoalveolar lavage fluid (BALF) was collected, and then the mice were sacrificed to collect their lung tissues. CD 4+ T cells were isolated from the control and asthmatic BALF samples. The expression levels of miRNA-451a in (B) CD 4+ T cells and (C) lung tissues were determined with real-time qPCR. The mRNA and protein levels of CDH11 were determined with (D) real-time qPCR, (E) western blot, and (F) immunofluorescent staining (bars, 50 μm). Data were expressed as mean ± S.D., and p < 0.05 was regarded statistically significant. *, **, *** indicated a p < 0.05, 0.01, 0.001.

Application: WB    Species: Mouse    Sample: lung tissues

Fig. 1. Opposite expression of miRNA-451a and CDH11 in the lung of asthma mouse pups. (A) New born mice were intraperitoneally injected with 10 μg ovalbumin (OVA) from postnatal day 5 (P5) to P10, and followed by 10-min daily challenge with 3% aerosolized OVA from P18 to P20. Twenty-four hours post the last OVA challenge (P21), the bronchoalveolar lavage fluid (BALF) was collected, and then the mice were sacrificed to collect their lung tissues. CD 4+ T cells were isolated from the control and asthmatic BALF samples. The expression levels of miRNA-451a in (B) CD 4+ T cells and (C) lung tissues were determined with real-time qPCR. The mRNA and protein levels of CDH11 were determined with (D) real-time qPCR, (E) western blot, and (F) immunofluorescent staining (bars, 50 μm). Data were expressed as mean ± S.D., and p < 0.05 was regarded statistically significant. *, **, *** indicated a p < 0.05, 0.01, 0.001.
2). BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo. MOLECULAR CARCINOGENESIS, 2017 (PubMed: 27770580) [IF=3.0]

Application: WB    Species: human    Sample: HepG2

Figure 6. Effect of BCL2L10 on its downstream gene expression profiles of human cancer pathway in HepG2 cells. (A) By human cancer pathway PCR array, ectopic expression of BCL2L10 up- or down-regulated several genes related to tumor proliferation, apoptosis, metastasis and angiogenesis. (B) Western blot was performed to confirm the downstream gene expression regulated by BCL2L10 in HepG2 cells. GAPDH was used as an internal control. (C) Schematic diagram of the molecular events for BCL2L10 function as a tumor suppressor through regulating cell cycle, proliferation, apoptosis metastasis and angiogenesis effectors.

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