PARD3 Antibody - #DF3368

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製品: PARD3 Antibody
カタログ: DF3368
タンパク質の説明: Rabbit polyclonal antibody to PARD3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 151 KD; 151kD(Calculated).
ユニプロット: Q8TEW0
RRID: AB_2835750

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(82%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
PARD3 Antibody detects endogenous levels of total PARD3.
RRID:
AB_2835750
引用形式: Affinity Biosciences Cat# DF3368, RRID:AB_2835750.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

agouti signaling protein; ASIP; Atypical PKC isotype specific interacting protein; Atypical PKC isotype-specific-interacting protein; Baz; bazooka; Coagulation factor II receptor-like 2; CTCL tumor antigen se2-5; F2rl2; PAR-3; par-3 family cell polarity regulator alpha; par-3 family cell polarity regulator; PAR3 alpha; par3; PAR3-alpha; PARD-3; Pard3; PARD3_HUMAN; PARD3A; Partitioning defective 3 homolog; Partitioning-defective 3 homolog; Partitioning-defective protein 3, C. elegans, homolog of; SE2-5L16; SE2-5LT1; SE2-5T2; Thrombin receptor-like 2;

免疫原

免疫原:
Uniprot:

Q8TEW0(PARD3_HUMAN) >>Visit HPA database.

遺伝子(ID):
PARD3(56288)
発現特異性:
Q8TEW0 PARD3_HUMAN:

Widely expressed.

タンパク質配列:
MKVTVCFGRTRVVVPCGDGHMKVFSLIQQAVTRYRKAIAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHVRRSSDPALIGLSTSVSDSNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYRSLPRDTSNWSNQFQRDNARSSLSASHPMVGKWLEKQEQDEDGTEEDNSRVEPVGHADTGLEHIPNFSLDDMVKLVEVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEHENLFRENDCIVRINDGDLRNRRFEQAQHMFRQAMRTPIIWFHVVPAANKEQYEQLSQSEKNNYYSSRFSPDSQYIDNRSVNSAGLHTVQRAPRLNHPPEQIDSHSRLPHSAHPSGKPPSAPASAPQNVFSTTVSSGYNTKKIGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLVGKSQEEVVSLLRSTKMEGTVSLLVFRQEDAFHPRELNAEPSQMQIPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKTNQDAMETLRRSMSTEGNKRGMIQLIVARRISKCNELKSPGSPPGPELPIETALDDRERRISHSLYSGIEGLDESPSRNAALSRIMGESGKYQLSPTVNMPQDDTVIIEDDRLPVLPPHLSDQSSSSSHDDVGFVTADAGTWAKAAISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSDASQLDFVKTRKSKSMDLGIADETKLNTVDDQKAGSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGDIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPAVDDDDEGMETLEEDTEESSRSGRESVSTASDQPSHSLERQMNGNQEKGDKTDRKKDKTGKEKKKDRDKEKDKMKAKKGMLKGLGDMFRFGKHRKDDKIEKTGKIKIQESFTSEEERIRMKQEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELMYGGVSSYEGSMALNARPQSPREGHMMDALYAQVKKPRNSKPSPVDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQPWPNARPATQSGRHSVSVEVQMQRQRQEERESSQQAQRQYSSLPRQSRKNASSVSQDSWEQNYSPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQMKKQPPSEGPSNYDSYKKVQDPSYAPPKGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
82
Xenopus
55
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TEW0 基板として

Site PTM Type Enzyme
K2 Acetylation
K22 Acetylation
S25 Phosphorylation
T32 Phosphorylation
T91 Phosphorylation
S94 Phosphorylation
T96 Phosphorylation
S100 Phosphorylation
Y119 Phosphorylation
S143 Phosphorylation
S144 Phosphorylation Q7KZI7 (MARK2)
S154 Phosphorylation
S156 Phosphorylation
S161 Phosphorylation
S166 Phosphorylation
T171 Phosphorylation
S174 Phosphorylation
T175 Phosphorylation
T176 Phosphorylation
K181 Methylation
S187 Phosphorylation
Y199 Phosphorylation
S201 Phosphorylation
S220 Phosphorylation
S221 Phosphorylation
S223 Phosphorylation
S225 Phosphorylation
K235 Ubiquitination
R294 Methylation
T298 Phosphorylation
S370 Phosphorylation
K374 Ubiquitination
Y377 Phosphorylation
Y378 Phosphorylation
S379 Phosphorylation
S380 Phosphorylation
S383 Phosphorylation
S386 Phosphorylation
Y388 Phosphorylation
S474 Phosphorylation
T481 Phosphorylation
S485 Phosphorylation
Y489 Phosphorylation
S523 Phosphorylation
S529 Phosphorylation
S533 Phosphorylation
T534 Phosphorylation
T579 Phosphorylation
S596 Phosphorylation
S598 Phosphorylation
S604 Phosphorylation
T654 Phosphorylation
S665 Phosphorylation
S667 Phosphorylation
T668 Phosphorylation
K672 Acetylation
S685 Phosphorylation
S692 Phosphorylation
S695 Phosphorylation
S715 Phosphorylation
S717 Phosphorylation
Y719 Phosphorylation
S720 Phosphorylation
S728 Phosphorylation
S730 Phosphorylation
S803 Phosphorylation
S807 Phosphorylation
S809 Phosphorylation
S827 Phosphorylation P41743 (PRKCI) , Q05513 (PRKCZ)
T833 Phosphorylation
S837 Phosphorylation
S840 Phosphorylation
S850 Phosphorylation
S852 Phosphorylation
S873 Phosphorylation Q7KZI7 (MARK2)
S875 Phosphorylation
S881 Phosphorylation
K885 Ubiquitination
S887 Phosphorylation
S888 Phosphorylation
S889 Phosphorylation
S892 Phosphorylation
T895 Phosphorylation
S924 Phosphorylation
Y933 Phosphorylation
T947 Phosphorylation
S958 Phosphorylation
S962 Phosphorylation O14965 (AURKA) , Q96GD4 (AURKB)
S967 Phosphorylation
S971 Phosphorylation
S973 Phosphorylation
K1005 Acetylation
K1007 Acetylation
K1013 Acetylation
K1014 Acetylation
S1046 Phosphorylation
Y1080 Phosphorylation
Y1098 Phosphorylation
Y1104 Phosphorylation
S1116 Phosphorylation
Y1127 Phosphorylation P07947 (YES1)
S1139 Phosphorylation
S1143 Phosphorylation
S1146 Phosphorylation
T1147 Phosphorylation
T1176 Phosphorylation
Y1177 Phosphorylation
S1178 Phosphorylation
S1196 Phosphorylation
S1198 Phosphorylation
Y1221 Phosphorylation
S1222 Phosphorylation
S1223 Phosphorylation
S1239 Phosphorylation
Y1244 Phosphorylation
S1245 Phosphorylation
Y1259 Phosphorylation
S1261 Phosphorylation
Y1269 Phosphorylation
Y1310 Phosphorylation
S1312 Phosphorylation
Y1313 Phosphorylation
Y1321 Phosphorylation
S1335 Phosphorylation
S1337 Phosphorylation
T1347 Phosphorylation
S1356 Phosphorylation

研究背景

機能:

Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Involved in Schwann cell peripheral myelination (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (By similarity). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.

PTMs:

Acetylated. Deacetylated by SIRT2, thereby inhibiting Schwann cell peripheral myelination.

Phosphorylation at Ser-827 by PRKCZ and PRKCI occurs at the most apical tip of epithelial cell-cell contacts during the initial phase of tight junction formation and may promote dissociation of the complex with PARD6. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2 (By similarity). Phosphorylation by AURKA at Ser-962 is required for the normal establishment of neuronal polarity.

細胞の位置付け:

Cytoplasm. Endomembrane system. Cell junction. Cell junction>Tight junction. Cell junction>Adherens junction. Cell membrane. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton.
Note: Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II. Colocalized with SIRT2 in internode region of myelin sheat (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed.

サブユニット構造:

Interacts (via PDZ 1 domain) with F11R/JAM1, PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. Interacts with PRCKI and CDH5. Interacts (via PDZ 3 domain) with PTEN (via C-terminus) (By similarity). Part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Interacts with LIMK2, AURKA and AURKB. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Directly interacts with TIAM1 and TIAM2. Interacts with ECT2, FBF1 and SIRT2. Interacts (via coiled-coil domain) with FRMD4A (By similarity). Found in a complex with PARD3, CYTH1 and FRMD4A (By similarity). Interacts with SAPCD2. Interacts with PRKCA.

タンパク質ファミリー:

Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.

The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.

Belongs to the PAR3 family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

参考文献

1). Discovery of the Natural Bibenzyl Compound Erianin in Dendrobium Inhibiting the Growth and EMT of Gastric Cancer through Downregulating the LKB1-SIK2/3-PARD3 Pathway. International journal of molecular sciences, 2024 (PubMed: 39063214) [IF=5.6]
2). Constitutive activation of β-catenin in odontoblasts induces aberrant pulp calcification in mouse incisors. JOURNAL OF MOLECULAR HISTOLOGY, 2021 (PubMed: 33689044) [IF=3.2]

Application: WB    Species: Mouse    Sample: calcification tissue

Fig. 6 Downregulation of polarity-related downstream molecules in incisor odontoblasts on day 13.5. a RT-qPCR quantification analy- sis of intercellular junction-related genes and polarity-related down- stream genes. CA-β-catenin mice displayed downregulated expres- sion levels of N-cadherin, ZO-1, Cdc42, Par3, Par6 and aPKC. b Western blot analysis of intercellular junction-related proteins and polarity-related downstream proteins. The fold activation data analy- sis is shown in (c). CA-β-catenin mice exhibited lower expression levels of N-cadherin, ZO-1, Cdc42, Par3, Par6 and aPKC. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6. (Color figure online)

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