DBF4 Antibody - #DF3462
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3462, RRID:AB_2835826.
折りたたみ／展開
Activator of S phase kinase; ASK; CHIF; Chiffon homolog A; DBF4; DBF4 homolog (S. cerevisiae); DBF4 homolog; DBF4 type zinc finger containing protein 1; DBF4 zinc finger A; DBF4 zinc finger; DBF4, S. cerevisiae, homolog of; DBF4-type zinc finger-containing protein 1; DBF4A; DBF4A_HUMAN; Dumbbell former 4, S. cerevisiae, homolog of; OTTHUMP00000161661; Protein DBF4 homolog A; ZDBF1; Zinc finger, DBF-type containing 1;
免疫原
Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
- Q9UBU7 DBF4A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UBU7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| K21 | Sumoylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| T273 | Phosphorylation | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| K408 | Sumoylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| T449 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| K455 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| S508 | Phosphorylation | Uniprot | |
| T510 | Phosphorylation | Uniprot | |
| T535 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| T553 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| K579 | Sumoylation | Uniprot | |
| S612 | Phosphorylation | Uniprot | |
| S613 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| T635 | Phosphorylation | Uniprot | |
| T638 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot |
研究背景
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Phosphorylation increases its interaction with PSIP1.
Nucleus.
Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6. Interacts (via IBM motifs) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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