PTTG1 Antibody - #AF0354

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製品: PTTG1 Antibody
カタログ: AF0354
タンパク質の説明: Rabbit polyclonal antibody to PTTG1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 30kDa; 22kD(Calculated).
ユニプロット: O95997
RRID: AB_2833520

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(88%), Bovine(89%), Sheep(89%), Rabbit(89%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
PTTG1 Antibody detects endogenous levels of total PTTG1.
RRID:
AB_2833520
引用形式: Affinity Biosciences Cat# AF0354, RRID:AB_2833520.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AW555095; C87862; Cut2; EAP 1; EAP1; ESP1 associated protein 1; Esp1-associated protein; hPTTG; MGC126883; MGC138276; Pds1; Pituitary tumor transforming 1; Pituitary tumor transforming protein 1; Pituitary tumor-transforming 1, isoform CRA_a; Pituitary tumor-transforming 1, isoform CRA_b; Pituitary tumor-transforming gene 1; Pituitary tumor-transforming gene 1 protein; PTTG 1; PTTG; PTTG1; PTTG1 protein; PTTG1_HUMAN; Pttg3; Securin; Tumor transforming 1; Tumor transforming protein 1; Tumor-transforming protein 1; TUTR 1; TUTR1;

免疫原

免疫原:
Uniprot:

O95997(PTTG1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PTTG1(9232)
発現特異性:
O95997 PTTG1_HUMAN:

Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.

タンパク質の説明:
securin Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53.
タンパク質配列:
MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLPLSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Bovine
89
Sheep
89
Rabbit
89
Pig
88
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95997 基板として

Site PTM Type Enzyme
Ubiquitination
A2 Acetylation
Y6 Phosphorylation
K9 Ubiquitination
T16 Phosphorylation
K25 Ubiquitination
S28 Phosphorylation
S31 Phosphorylation
K33 Ubiquitination
S39 Phosphorylation
S42 Phosphorylation
T43 Phosphorylation
K48 Acetylation
K48 Ubiquitination
T60 Phosphorylation
K62 Ubiquitination
T66 Phosphorylation
S87 Phosphorylation
S89 Phosphorylation
K101 Ubiquitination
Y111 Phosphorylation
S165 Phosphorylation P27361 (MAPK3) , P06493 (CDK1) , P28482 (MAPK1)
S171 Phosphorylation
S181 Phosphorylation
S183 Phosphorylation
S184 Phosphorylation

研究背景

機能:

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

PTMs:

Phosphorylated at Ser-165 by CDK1 during mitosis.

Phosphorylated in vitro by ds-DNA kinase.

Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.

サブユニット構造:

Interacts with RPS10 and DNAJA1 (By similarity). Interacts with the caspase-like ESPL1, and prevents its protease activity probably by covering its active site. Interacts with TP53 and blocks its activity probably by blocking its binding to DNA. Interacts with the Ku 70 kDa subunit of ds-DNA kinase. Interacts with PTTG1IP.

タンパク質ファミリー:

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.

The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thioester with the acceptor lysine residue.

Belongs to the securin family.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

参考文献

1). Comparison of the major cell populations among osteoarthritis, Kashin–Beck disease and healthy chondrocytes by single-cell RNA-seq analysis. Cell Death & Disease, 2021 (PubMed: 34045450) [IF=9.0]

Application: IHC    Species: Human    Sample: cartilage tissues

Fig. 4 Single-cell RNA-sequencing analysis of the cell population in healthy controls. A Gene expression plots of CENPW, PTTG1 and BIRC5. Dot colour corresponds to the level of gene expression in each cell. t-SNE, t-distributed stochastic neighbour embedding. B Representative immunohistochemistry staining of CENPW and PTTG1 in healthy cartilage tissues. Scale bar, left, 500 μm; right, 50 μm, and quantification of positive cells of different areas (up, middle, deep) in healthy cartilage tissues displayed by box plot (n = 5). *p < 0.05. C, D Monocle pseudotime trajectory showing the progression of KBD, OA and the control. E The expression of the genes in a branch-dependent manner. Each row indicates the standardised kinetic curves of a gene. The centre of the heatmap shows the kinetic curve value at the root of the trajectory. From the centre to the left of the heatmap, the kinetic curve progresses from the root along the trajectory to fate 1. Starting from the right, the curve from the root to fate 2.

Application: IHC    Species: human    Sample: cartilage

Fig. 4 | Single-cell RNA-sequencing analysis of the cell population in healthy controls.B Representative immunohistochemistry staining of CENPW and PTTG1 in healthy cartilage tissues. Scale bar, left, 500 μm; right, 50 μm, and quantification of positive cells of different areas (up, middle, deep) in healthy cartilage tissues displayed by box plot (n = 5). *p < 0.05.
2). SIRT1 suppresses pituitary tumor progression by downregulating PTTG1 expression. ONCOLOGY REPORTS, 2022 (PubMed: 35730625) [IF=4.2]

Application: WB    Species: Human    Sample:

Figure 5. - H3K9ac reader BRD4 is required for PTTG1 expression. (A) Bar plot revealing the relative mRNA expression. *P

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