CDK11 Antibody - #DF3475

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製品: CDK11 Antibody
カタログ: DF3475
タンパク質の説明: Rabbit polyclonal antibody to CDK11
アプリケーション: WB IF/ICC
反応性: Human
分子量: 90 KD; 91kD,93kD(Calculated).
ユニプロット: Q9UQ88 | P21127
RRID: AB_2835838

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
CDK11 Antibody detects endogenous levels of total CDK11.
RRID:
AB_2835838
引用形式: Affinity Biosciences Cat# DF3475, RRID:AB_2835838.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CD11A_HUMAN; CDC2L2; CDC2L3; CDK11; CDK11 p110; CDK11 p46; CDK11 p58; CDK11A; Cell division cycle 2 like 2 (PITSLRE proteins); Cell division cycle 2-like protein kinase 2; Cell division protein kinase 11A; Cyclin-dependent kinase 11A; Galactosyltransferase-associated protein kinase p58/GTA; p58GTA; PITSLRE; PITSLRE B; PITSLRE protein kinase beta; PITSLRE serine/threonine-protein kinase CDC2L2; PITSLREB; CDC2L1; CDK11; Cell division cycle 2-like protein kinase 1; Cell division protein kinase 11B; CLK 1; EC=2.7.11.22; Galactosyltransferase associated protein kinase p58/GTA; ITSLRE serine/threonine protein kinase CDC2L1; p58 CLK 1; PITSLREA; PK58;

免疫原

免疫原:
Uniprot:

Q9UQ88(CD11A_HUMAN) >>Visit HPA database.

P21127(CD11B_HUMAN) >>Visit HPA database.

遺伝子(ID):
CDK11A(728642) | CDK11B(984)
発現特異性:
Q9UQ88 CD11A_HUMAN:

Expressed ubiquitously. Some evidence of isoform-specific tissue distribution.

P21127 CD11B_HUMAN:

Expressed ubiquitously. Some evidence of isoform-specific tissue distribution.

タンパク質配列:
MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKDEKRKEKWKHARVKEREHERRKRHREEQDKARREWERQKRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMREDYSDKVKASHWSRSPPRPPRERFELGDGRKPGEARPAPAQKPAQLKEEKMEERDLLSDLQDISDSERKTSSAESSSAESGSGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEERENENHLLVVPESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALLPIELKQELPKYLPALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVVVTQWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGNSEIDQINKVFKELGTPSEKIWPGYSELPVVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLKF

MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHRMEITIRNSPYRREDSMEDRGEEDDSLAIKPPQQMSRKEKAHHRKDEKRKEKRRHRSHSAEGGKHARVKEKEREHERRKRHREEQDKARREWERQKRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMREDYSDKVKASHWSRSPPRPPRERFELGDGRKPGEARPAPAQKPAQLKEEKMEERDLLSDLQDISDSERKTSSAESSSAESGSGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEERENENHLLVVPESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALSPIELKQELPKYLPALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVVVTLWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEIDQINKVFKDLGTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLKF

PTMs - Q9UQ88/P21127 基板として

Site PTM Type Enzyme
K11 Sumoylation
K11 Ubiquitination
K20 Sumoylation
K20 Ubiquitination
K29 Sumoylation
S47 Phosphorylation
T61 Phosphorylation
S65 Phosphorylation
Y67 Phosphorylation
S72 Phosphorylation
K101 Acetylation
K123 Acetylation
S200 Phosphorylation
Y210 Phosphorylation
S211 Phosphorylation
K213 Acetylation
S217 Phosphorylation
S220 Phosphorylation
S222 Phosphorylation
K254 Sumoylation
S265 Phosphorylation
S271 Phosphorylation
S273 Phosphorylation
S346 Phosphorylation
S369 Phosphorylation
S372 Phosphorylation
S398 Phosphorylation
K407 Sumoylation
K412 Ubiquitination
S422 Phosphorylation
T436 Phosphorylation
Y437 Phosphorylation
Y441 Phosphorylation
K444 Acetylation
K446 Acetylation
K447 Acetylation
K447 Sumoylation
K447 Ubiquitination
K455 Acetylation
K455 Sumoylation
K455 Ubiquitination
K463 Ubiquitination
K479 Sumoylation
K479 Ubiquitination
K519 Ubiquitination
K539 Ubiquitination
K552 Sumoylation
K552 Ubiquitination
K565 Ubiquitination
Y575 Phosphorylation
S577 Phosphorylation
Y582 Phosphorylation
T583 Phosphorylation
T588 Phosphorylation
K637 Sumoylation
K637 Ubiquitination
K640 Ubiquitination
K648 Ubiquitination
K660 Ubiquitination
K661 Sumoylation
K661 Ubiquitination
T663 Phosphorylation
S701 Phosphorylation
K707 Sumoylation
K707 Ubiquitination
T714 Phosphorylation
K729 Sumoylation
K729 Ubiquitination
T739 Phosphorylation
S740 Phosphorylation O96017 (CHEK2)
Y750 Phosphorylation
S751 Phosphorylation
T761 Phosphorylation
T766 Phosphorylation
S773 Phosphorylation
S780 Phosphorylation
K782 Methylation
K782 Sumoylation
K782 Ubiquitination
Site PTM Type Enzyme
K11 Sumoylation
K11 Ubiquitination
K20 Sumoylation
K20 Ubiquitination
K29 Sumoylation
S43 Phosphorylation
S47 Phosphorylation
T61 Phosphorylation
S65 Phosphorylation
Y67 Phosphorylation
S72 Phosphorylation
K101 Acetylation
S113 Phosphorylation
S115 Phosphorylation
K135 Acetylation
S212 Phosphorylation
Y222 Phosphorylation
S223 Phosphorylation
K225 Acetylation
S229 Phosphorylation
S232 Phosphorylation
S234 Phosphorylation
K266 Sumoylation
S277 Phosphorylation
S283 Phosphorylation
S285 Phosphorylation
S358 Phosphorylation
S381 Phosphorylation
S384 Phosphorylation
T395 Phosphorylation
T402 Phosphorylation
Y406 Phosphorylation
S410 Phosphorylation
S414 Phosphorylation
K419 Sumoylation
K424 Ubiquitination
S434 Phosphorylation
T448 Phosphorylation
Y449 Phosphorylation
Y453 Phosphorylation
K456 Acetylation
K458 Acetylation
K459 Acetylation
K459 Sumoylation
K459 Ubiquitination
K467 Acetylation
K467 Sumoylation
K467 Ubiquitination
K475 Ubiquitination
S482 Phosphorylation
T488 Phosphorylation
K491 Sumoylation
K491 Ubiquitination
K531 Ubiquitination
K551 Ubiquitination
K564 Sumoylation
K564 Ubiquitination
K577 Ubiquitination
Y587 Phosphorylation
S589 Phosphorylation
Y594 Phosphorylation
T595 Phosphorylation P50613 (CDK7)
T600 Phosphorylation
K641 Sumoylation
K641 Ubiquitination
K649 Sumoylation
K649 Ubiquitination
K652 Sumoylation
K652 Ubiquitination
K660 Ubiquitination
K672 Ubiquitination
K673 Sumoylation
K673 Ubiquitination
T675 Phosphorylation
S713 Phosphorylation
K719 Sumoylation
K719 Ubiquitination
T726 Phosphorylation Q9UQ88 (CDK11A)
K741 Sumoylation
K741 Ubiquitination
T751 Phosphorylation
S752 Phosphorylation O96017 (CHEK2)
Y762 Phosphorylation
S763 Phosphorylation
T773 Phosphorylation
T778 Phosphorylation
S785 Phosphorylation
S792 Phosphorylation
K794 Methylation
K794 Sumoylation
K794 Ubiquitination

PTMs - Q9UQ88/P21127 酵素として

Substrate Site Source
O95238 (SPDEF) S238 Uniprot
O95238 (SPDEF) S242 Uniprot
O95238 (SPDEF) S243 Uniprot
P21127-12 (CDK11B) T370 Uniprot
P21127 (CDK11B) T726 Uniprot
Substrate Site Source
O00303 (EIF3F) S46 Uniprot
O00303 (EIF3F) T119 Uniprot

研究背景

機能:

Appears to play multiple roles in cell cycle progression, cytokinesis and apoptosis. The p110 isoforms have been suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. The p58 isoform may act as a negative regulator of normal cell cycle progression.

PTMs:

During apoptosis, induced by Fas or tumor necrosis factor, specific CKD11 p110 isoforms are cleaved by caspases to produce a protein (p110C) that contains the C-terminal kinase domain of the CDK11 proteins.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed ubiquitously. Some evidence of isoform-specific tissue distribution.

サブユニット構造:

The cleaved p110 isoform, p110C, binds to the serine/threonine kinase PAK1. The p58 isoform but not the p110 isoform or p110C interacts with CCND3. The p110 isoforms are found in large molecular weight complexes containing CCNL1 and SFRS7.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

機能:

Plays multiple roles in cell cycle progression, cytokinesis and apoptosis. Involved in pre-mRNA splicing in a kinase activity-dependent manner. Isoform 7 may act as a negative regulator of normal cell cycle progression.

PTMs:

During FAS- or TNF-induced apoptosis, isoform SV9 is cleaved by caspases to produce p110C, a fragment that contains the C-terminal kinase domain.

Phosphorylation at Ser-115 creates a binding site for 14-3-3 proteins. p110C can be autophosphorylated.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed ubiquitously. Some evidence of isoform-specific tissue distribution.

サブユニット構造:

Cleaved isoform SV9 (p110C) binds to the serine/threonine kinase PAK1 and RANBP9. p110C interacts with RNPS1. Isoform 7, but not isoform SV9, nor its cleavage product p110C, interacts with CCND3. Interacts with CCNL1 and CCNL2. Forms complexes with pre-mRNA-splicing factors, including at least SRSF1, SRSF2 AND SRSF7/SLU7. Interacts with isoform 5 of MYO18A.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

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