DYR1B Antibody - #DF3484
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3484, RRID:AB_2835847.
折りたたみ／展開
AOMS3; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B; Dual specificity tyrosine phosphorylation regulated kinase 1B; Dual specificity tyrosine-phosphorylation-regulated kinase 1B; DYR 1B; DYR1A; DYR1B; DYR1B_HUMAN; DYRK 1B; Dyrk1b; Minibrain related kinase; Minibrain-related kinase; MIRK; Mirk protein kinase;
免疫原
Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
- Q9Y463 DYR1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y463 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S49 | Phosphorylation | Uniprot | |
| K57 | Acetylation | Uniprot | |
| Y63 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| Y271 | Phosphorylation | Q9Y463 (DYRK1B) | Uniprot |
| Y273 | Phosphorylation | Q9Y463 (DYRK1B) | Uniprot |
| S276 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Q9Y463 (DYRK1B) , P28482 (MAPK1) | Uniprot |
| Y485 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot |
PTMs - Q9Y463 酵素として
| Substrate | Site | Source |
|---|---|---|
| O75533 (SF3B1) | T434 | Uniprot |
| P20823 (HNF1A) | S249 | Uniprot |
| P24385 (CCND1) | T286 | Uniprot |
| P24385 (CCND1) | T288 | Uniprot |
| P38936 (CDKN1A) | S153 | Uniprot |
| P46527 (CDKN1B) | S10 | Uniprot |
| Q02363 (ID2) | T27 | Uniprot |
| Q99801 (NKX3-1) | S185 | Uniprot |
| Q9UQL6 (HDAC5) | S279 | Uniprot |
| Q9Y463 (DYRK1B) | Y271 | Uniprot |
| Q9Y463 (DYRK1B) | Y273 | Uniprot |
| Q9Y463 (DYRK1B) | S421 | Uniprot |
研究背景
Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase (G6PC).
Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.
Nucleus.
Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
Dimer. Interacts with DCOHM, MAP2K3/MKK3, RANBP9 and TCF1/HNF1A. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Interacts with DCAF7.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
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