製品: RPS20 Antibody
カタログ: DF3681
タンパク質の説明: Rabbit polyclonal antibody to RPS20
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 13 KD; 13kD(Calculated).
ユニプロット: P60866
RRID: AB_2835891

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
RPS20 Antibody detects endogenous levels of total RPS20.
RRID:
AB_2835891
引用形式: Affinity Biosciences Cat# DF3681, RRID:AB_2835891.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

40S ribosomal protein S20; Ribosomal protein S20; RPS 20; RpS20; RS20_HUMAN; S20;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60866 基板として

Site PTM Type Enzyme
K4 Acetylation
K4 Ubiquitination
T6 Phosphorylation
K8 Acetylation
K8 Methylation
K8 Sumoylation
K8 Ubiquitination
T9 Phosphorylation
R19 Methylation
K30 Ubiquitination
S31 Phosphorylation
K34 Acetylation
K34 Ubiquitination
R41 Methylation
K44 Acetylation
K44 Ubiquitination
K49 Ubiquitination
K51 Ubiquitination
T58 Phosphorylation
K59 Ubiquitination
K67 Ubiquitination
K75 Ubiquitination
T76 Phosphorylation
R79 Methylation
S93 Phosphorylation
S95 Phosphorylation
K99 Ubiquitination

研究背景

PTMs:

Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the 40S small ribosomal subunit.

タンパク質ファミリー:

Belongs to the universal ribosomal protein uS10 family.

研究領域

· Genetic Information Processing > Translation > Ribosome.

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