Cytochrome P450 19A1 Antibody - #DF3564

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製品: Cytochrome P450 19A1 Antibody
カタログ: DF3564
タンパク質の説明: Rabbit polyclonal antibody to Cytochrome P450 19A1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 53 KD; 58kD(Calculated).
ユニプロット: P11511
RRID: AB_2835936

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HPLC Report
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(83%), Bovine(88%), Sheep(88%), Rabbit(100%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
Cytochrome P450 19A1 Antibody detects endogenous levels of total Cytochrome P450 19A1.
RRID:
AB_2835936
引用形式: Affinity Biosciences Cat# DF3564, RRID:AB_2835936.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ARO; ARO1; Aromatase; CP19A_HUMAN; CPV1; CYAR; CYP19; Cyp19a1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Cytochrome P450, family 19, subfamily A, polypeptide 1; Cytochrome P450, subfamily XIX (aromatization of androgens); Estrogen synthase; Estrogen synthetase; Flavoprotein linked monooxygenase; MGC104309; Microsomal monooxygenase; OTTHUMP00000162543; OTTHUMP00000198350; P 450AROM;

免疫原

免疫原:
Uniprot:

P11511(CP19A_HUMAN) >>Visit HPA database.

遺伝子(ID):
CYP19A1(1588)
発現特異性:
P11511 CP19A_HUMAN:

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

タンパク質配列:
MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGIGSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPELWKTTRPFFMKALSGPGLVRMVTVCAESLKTHLDRLEEVTNESGYVDVLTLLRRVMLDTSNTLFLRIPLDESAIVVKIQGYFDAWQALLIKPDIFFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFATELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDIQKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAKNVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEMIFTPRNSDRCLEH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
88
Sheep
88
Dog
88
Pig
83
Horse
50
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11511 基板として

Site PTM Type Enzyme
S118 Phosphorylation
T162 Phosphorylation
S167 Phosphorylation
T201 Phosphorylation
Y220 Phosphorylation
Y241 Phosphorylation
Y361 Phosphorylation P12931 (SRC)
T392 Phosphorylation
Y441 Phosphorylation
T484 Phosphorylation
S497 Phosphorylation

研究背景

機能:

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

PTMs:

Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1).

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Microsome membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

タンパク質ファミリー:

Belongs to the cytochrome P450 family.

研究領域

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

参考文献

1). Exposure to 1-nitropyrene after weaning induces anxiety-like behavior partially by inhibiting steroid hormone synthesis in prefrontal cortex. Journal of hazardous materials, 2024 (PubMed: 38889457) [IF=13.6]
2). Naringenin improves ovarian health by reducing the serum androgen and eliminating follicular cysts in letrozole-induced polycystic ovary syndrome in the Sprague Dawley rats. Phytotherapy research : PTR, 2023 (PubMed: 37165686) [IF=7.2]
3). Deubiquitinase UCHL1 regulates estradiol synthesis by stabilizing voltage-dependent anion channel 2. Journal of Biological Chemistry, 2023 (PubMed: 37797697) [IF=4.8]

Application: WB    Species: porcine    Sample: GCs

Figure 2 UCHL1 promotes E2synthesis in GCs.A, overexpression efficiency of UCHL1 after transfection with pcDNA3.1-UCHL1 compared to pcDNA3.1. B, E2 levels were measured using ELISA. Culture supernatants were collected 24 h after pcDNA3.1-UCHL1 and pcDNA3.1 treatments. C and H, RT-qPCR detected critical genes in E2 synthesis, including StAR, CYP11A1, 3β-HSD, and CYP19A1. D and I, Western blot analysis of critical proteins in E2 synthesis. E and J, quantification of the Western blot analysis. F, inhibitory efficiency after transfection with si-UCHL1 compared with NC. G, E2 concentrations were measured using ELISA. Culture supernatants were collected 24 h after si-UCHL1 and NC treatments. Data are means ± SEMs of three independent experiments; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. GC, granulosa cell; UCHL1, ubiquitin C-terminal hydrolase L1.
4). Characterization of VDR and CYP27B1 expression in the endometrium during the menstrual cycle before embryo transfer: implications for endometrial receptivity. Reproductive Biology and Endocrinology, 2020 (PubMed: 32183826) [IF=4.4]

Application: IHC    Species: human    Sample: endometrial

Fig. 3| Immunohistochemistry localization of VDR (left column), HOXA10 (second column), CYP27B1 third column), and CYP19 (right column) during the secretory phase of the menstrual cycle in vitamin D deficient (top row), insufficient (second row), and replete (third row) patients. No signal was detected in the negative control sections (bottom row). All parts: magnification × 400, scale bar 50 μm

Application: WB    Species: human    Sample: endometrial

Fig. 4 |Western blot for VDR, CYP27B1, HOXA10, and CYP19 during the proliferative (a) and secretory (b) phases of the menstrual cycle
5). ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells. Molecular and Cellular Endocrinology, 2023 (PubMed: 37769867) [IF=4.1]

Application: WB    Species: Human    Sample: granulosa cells

Fig. 4. ZDHHC17 plays a role in the conversion of androgen to estrogen in GCs. (A) GRM02 cells were transfected with scramble (Scr) or Zdhhc17-specific siRNAs and then treated with DHT (100 nM) for 24 h. Cell lysates were collected to determine the mRNA expression level of Cyp19a1. (B) GRM02 cells were transfected with Zdhhc17-specific siRNAs, and cell lysates were collected to determine the protein expression level of CYP19A1. (C–E) GRM02 cells were transfected with scramble (Scr) or Zdhhc17-specific siRNAs and then supplemented with testosterone (10 ng/mL) for 24 h. Cell supernatants were collected to determine the estradiol levels (C) produced per 1 × 105 cells, testosterone levels (D) produced per 1 × 105 cells and estradiol to testosterone ratios (E). (F) CYP19A1 and ZDHHC17 protein levels in mouse ovarian tissues from the control and DHEA groups (n = 5 mice per group). In (A–E), data are presented as the mean ± SEM from three independent experiments, two-way ANOVA: ns indicates not significant, *P < 0.05, **P < 0.01. In (F), data are presented as the mean ± SD from five mice, unpaired two-tailed Student's t-test: **P < 0.01 compared with the control group.
6). Long-term exposure to the mixture of phthalates induced male reproductive toxicity in rats and the alleviative effects of quercetin. Toxicology and applied pharmacology, 2024 (PubMed: 38218207) [IF=3.8]
7). Estrogen deficiency exacerbates learning and memory deficits associated with glucose metabolism disorder in APP/PS1 double transgenic female mice. Genes & Diseases, 2022 (PubMed: 35873026)

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