Cytochrome P450 2E1 Antibody - #DF3578
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3578, RRID:AB_2835950.
折りたたみ／展開
4-nitrophenol 2-hydroxylase; CP2E1_HUMAN; CPE1; CYP2E; Cyp2e1; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 isozyme 3A; Cytochrome P450 LM3A; Cytochrome P450, family 2, subfamily e, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450, subfamily IIE; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; EC 1.14.13.-; EC 1.14.13.n7; Ethanol-inducible P450; Flavoprotein-linked monooxygenase; MGC133529; Microsomal monooxygenase; OTTHUMP00000020813; OTTHUMP00000046571; P450 ALC; P450-J; P450C2E; P450J; P450RLM6; Xenobiotic monooxygenase;
免疫原
- P05181 CP2E1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSALGVTVALLVWAAFLLLVSMWRQVHSSWNLPPGPFPLPIIGNLFQLELKNIPKSFTRLAQRFGPVFTLYVGSQRMVVMHGYKAVKEALLDYKDEFSGRGDLPAFHAHRDRGIIFNNGPTWKDIRRFSLTTLRNYGMGKQGNESRIQREAHFLLEALRKTQGQPFDPTFLIGCAPCNVIADILFRKHFDYNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPSFLHYLPGSHRKVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGCIPPRYKLCVIPRS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P05181 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S56 | Phosphorylation | Uniprot | |
| T58 | Phosphorylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| T121 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| T132 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| K461 | Ubiquitination | Uniprot |
研究背景
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).
Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein. Mitochondrion inner membrane>Peripheral membrane protein.
Note: Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.
Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.
Belongs to the cytochrome P450 family.
研究領域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Cancers: Overview > Chemical carcinogenesis.
· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.
· Metabolism > Lipid metabolism > Arachidonic acid metabolism.
· Metabolism > Lipid metabolism > Linoleic acid metabolism.
· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
· Metabolism > Global and overview maps > Metabolic pathways.
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