IL17RA Antibody - #DF3602

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製品: IL17RA Antibody
カタログ: DF3602
タンパク質の説明: Rabbit polyclonal antibody to IL17RA
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 90 KD; 96kD(Calculated).
ユニプロット: Q96F46
RRID: AB_2835974

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(82%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
IL17RA Antibody detects endogenous levels of total IL17RA.
RRID:
AB_2835974
引用形式: Affinity Biosciences Cat# DF3602, RRID:AB_2835974.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CANDF5; CD217; CD217 antigen; CDw217; CTLA8; HIL 17R; hIL17R; I17RA_HUMAN; IL 17 receptor A; IL 17 receptor; IL 17RA; IL-17 receptor A; IL-17RA; IL17; IL17A receptor; IL17R; IL17RA; IMD51; Interleukin 17 receptor A; Interleukin-17 receptor A; MGC10262;

免疫原

免疫原:

A synthesized peptide derived from human IL17RA, corresponding to a region within N-terminal amino acids.

Uniprot:

Q96F46(I17RA_HUMAN) >>Visit HPA database.

遺伝子(ID):
IL17RA(23765)
発現特異性:
Q96F46 I17RA_HUMAN:

Widely expressed.

タンパク質配列:
MGAARSPPSAVPGPLLGLLLLLLGVLAPGGASLRLLDHRALVCSQPGLNCTVKNSTCLDDSWIHPRNLTPSSPKDLQIQLHFAHTQQGDLFPVAHIEWTLQTDASILYLEGAELSVLQLNTNERLCVRFEFLSKLRHHHRRWRFTFSHFVVDPDQEYEVTVHHLPKPIPDGDPNHQSKNFLVPDCEHARMKVTTPCMSSGSLWDPNITVETLEAHQLRVSFTLWNESTHYQILLTSFPHMENHSCFEHMHHIPAPRPEEFHQRSNVTLTLRNLKGCCRHQVQIQPFFSSCLNDCLRHSATVSCPEMPDTPEPIPDYMPLWVYWFITGISILLVGSVILLIVCMTWRLAGPGSEKYSDDTKYTDGLPAADLIPPPLKPRKVWIIYSADHPLYVDVVLKFAQFLLTACGTEVALDLLEEQAISEAGVMTWVGRQKQEMVESNSKIIVLCSRGTRAKWQALLGRGAPVRLRCDHGKPVGDLFTAAMNMILPDFKRPACFGTYVVCYFSEVSCDGDVPDLFGAAPRYPLMDRFEEVYFRIQDLEMFQPGRMHRVGELSGDNYLRSPGGRQLRAALDRFRDWQVRCPDWFECENLYSADDQDAPSLDEEVFEEPLLPPGTGIVKRAPLVREPGSQACLAIDPLVGEEGGAAVAKLEPHLQPRGQPAPQPLHTLVLAAEEGALVAAVEPGPLADGAAVRLALAGEGEACPLLGSPGAGRNSVLFLPVDPEDSPLGSSTPMASPDLLPEDVREHLEGLMLSLFEQSLSCQAQGGCSRPAMVLTDPHTPYEEEQRQSVQSDQGYISRSSPQPPEGLTEMEEEEEEEQDPGKPALPLSPEDLESLRSLQRQLLFRQLQKNSGWDTMGSESEGPSA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
82
Chicken
50
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Receptor for IL17A. Receptor for IL17F. Binds to IL17A with higher affinity than to IL17F. Binds IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RC. Also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC. Receptor for IL17C as part of a heterodimeric complex with IL17RE. Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6.

PTMs:

Glycosylated.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

参考文献

1). Gingerenone A Attenuates Ulcerative Colitis via Targeting IL-17RA to Inhibit Inflammation and Restore Intestinal Barrier Function. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38639442) [IF=15.1]

Application: WB    Species: Mouse    Sample:

Figure 4. Effect of GA on regulating IL‐17RA signaling and downstream signaling in DSS‐induced colitis mice. A) KEGG analysis. B) Heatmap of IL‐17RA signaling and downstream signaling genes. C) The fold change (fc), p value and false discovery rate (FDR) of IL‐17 signaling key genes. D) Circos represents different genes and different groups, the innermost lines showed the connections between the genes and groups. The thickness of the lines indicates the expressions levels of genes. E–G) Western blot analysis for IL‐17 signaling and downstream signaling proteins in colonic tissues of colitis mice. All data are expressed as mean ± S.E.M (n = 3). *P < 0.05 and **P < 0.01 compared with the control group, #P < 0.05 and ## P < 0.01 compared with the DSS group.
2). Alhagi camelorum seed polysaccharide alleviates methylglyoxal-induced skin damage via antioxidant and anti-inflammatory actions. International journal of biological macromolecules, 2025 (PubMed: 40311972) [IF=7.7]
3). IL-17a promotes hepatocellular carcinoma by increasing FAP expression in hepatic stellate cells via activation of the STAT3 signaling pathway. Cell death discovery, 2024 (PubMed: 38740736) [IF=7.0]
4). Development and Validation of a 7-Gene Inflammatory Signature Forecasts Prognosis and Diverse Immune Landscape in Lung Adenocarcinoma. Frontiers in Molecular Biosciences, 2022 (PubMed: 35372503) [IF=5.0]

Application: WB    Species: human    Sample: bronchial epithelial cell line (16-HBS) and lung adenocarcinoma cell line (A549）

FIGURE 10 | Protein expression of seven model genes. (A–C) Immunohistochemical staining images of MMP14 (A), PCDH7 (B), and LAMP3 (C) from the HPA database. (D) Western blotting results shows the protein expression of BTG2, TLR2, CCL20, and IL7R in a normal bronchial epithelial cell line (16-HBS) and lung adenocarcinoma cell line (A549).
5). Fuyuan Huoxue decoction ameliorates CFA-induced pain by modulating chemokine and IL-17 signaling pathways. Journal of ethnopharmacology, 2026 (PubMed: 41237869) [IF=4.8]
6). Cornuside protects against ischemic stroke in rats by suppressing the IL-17F/TRAF6/NF-κB pathway via the brain-gut axis. Experimental neurology, 2024 (PubMed: 38169196) [IF=4.6]
7). FOSL1 promotes keratinocyte migration and wound repair by modulating the IL17 signaling pathway. Scientific reports, 2025 (PubMed: 40355666) [IF=3.8]

Application: WB    Species: Mouse    Sample: traumatic epidermal tissue

Fig. 2 FOSL1 was upregulated in wound tissues. (A) Macroscopy view of wound healing on days 0, 1, 2, 3, 7, 14 mice. Scale bar: 1 mm. (B) A mouse back full-thickness skin resection trauma model in days 0, 1, 2, 3,7, The FOSL1 mRNA expression was determined by qPCR in 14. (C) A mouse back full-thickness skin resection trauma model in days 0, 1, 2, 3, 7, 14 FOSL1 protein expression was determined by Western Blot. (D) Quantification of C. All bars represent mean ± SD (n = 6). (E) qPCR detection of IL17A and IL17RA mRNA expression in traumatic epidermal tissue. (F, G) Western Blot test for the expression of IL17A and IL17RA proteins in traumatic epidermal tissue; Quantification of F. All bars represent mean ± SD (n = 3). *, P 
8). Moxibustion Enhances Ovarian Function by Inhibiting the Th17/IL-17 Pathway and Regulating Gut Microbiota in POI Rats. American journal of reproductive immunology (New York, N.Y. : 1989), 2025 (PubMed: 40322835) [IF=2.5]
9). Prolonged neoadjuvant hormonal therapy enhances IL-17 A/STAT3-mediated inflammation in prostate cancer. Discover oncology, 2025 (PubMed: 41236589) [IF=2.2]

Application: IHC    Species: human    Sample: prostate cancer tissue

Fig. 2 Immunohistochemical staining and scoring of prostate cancer tissue and para-carcinoma tissue after NHT(×400). A IL-17 A in prostate cancer tissue(×400) B. IL-17 A in para-carcinoma tissue(×400) C. IL-17RA in prostate cancer tissue (×400) D. IL-17RA in para-carcinoma tissue(×400) E. IL-17RC in prostate cancer tissue(×400) F. IL-17RC in para-carcinoma tissue(×400) G. IL-17 A in PCa was significantly higher than that in NP (P 
10). IL-17A exacerbates cisplatin-based resistance of OVCA via upregulating the expression of ABCG2 and MDR1 through Gli1-mediated Hh signaling. Oncotarget, 2016

Application: WB    Species: Human    Sample: A2780

Figure 1: rhIL-17A has no effect on OVCA cell proliferation. A. Protein levels of IL-17RA in five human OVCA cell lines, including A2780, OVCAR3, SKOV3, HO8910, HO8910/PM, and a mouse OVCA cell line ID8 analyzed by western blotting. Protein lysates from OVCA cells were prepared and analyzed by western blotting. Representative result from three independent experiments was showed. B. rhIL-17A had no effect on the proliferation of A2780 and OVCAR3 cells at given concentration (0-100ng/ml) at designated time points (12, 24, 48, 72h) by MTT assay. Data represent means±SD from three independent experiments.
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