Mnk1 Antibody - #AF0373
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0373, RRID:AB_2833534.
折りたたみ／展開
MAP kinase interacting kinase 1; MAP kinase interacting serine/threonine kinase 1; MAP kinase signal integrating kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal integrating kinase 1; MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1; mknk1; MKNK1_HUMAN; MNK 1; Mnk1;
免疫原
- Q9BUB5 MKNK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BUB5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S39 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| Y60 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| T255 | Phosphorylation | Q16539 (MAPK14) , P28482 (MAPK1) | Uniprot |
| K332 | Ubiquitination | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| T385 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S393 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| R455 | Methylation | Uniprot | |
| R459 | Methylation | Uniprot | |
| S460 | Phosphorylation | Uniprot |
PTMs - Q9BUB5 酵素として
| Substrate | Site | Source |
|---|---|---|
| O43597 (SPRY2) | S112 | Uniprot |
| O43597 (SPRY2) | S121 | Uniprot |
| P06730 (EIF4E) | S209 | Uniprot |
| P09651 (HNRNPA1) | S192 | Uniprot |
| P09651 (HNRNPA1) | S362 | Uniprot |
| P09651 (HNRNPA1) | S363 | Uniprot |
| P09651 (HNRNPA1) | S364 | Uniprot |
| P23246 (SFPQ) | S8 | Uniprot |
| P23246 (SFPQ) | S283 | Uniprot |
| P47712 (PLA2G4A) | S727 | Uniprot |
| Q9NP80 (PNPLA8) | S511 | Uniprot |
研究背景
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.
Cytoplasm.
Cytoplasm. Nucleus.
Ubiquitous.
Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.