EXO1 Antibody - #DF3615
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3615, RRID:AB_2835987.
折りたたみ／展開
exo1; EXO1_HUMAN; ExoI; Exonuclease 1; Exonuclease I; Exonuclease1; HEX1; hExo I; hExo1; hExoI; Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1;
免疫原
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
- Q9UQ84 EXO1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGIQGLLQFIKEASEPIHVRKYKGQVVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSHGIKPILVFDGCTLPSKKEVERSRRERRQANLLKGKQLLREGKVSEARECFTRSINITHAMAHKVIKAARSQGVDCLVAPYEADAQLAYLNKAGIVQAIITEDSDLLAFGCKKVILKMDQFGNGLEIDQARLGMCRQLGDVFTEEKFRYMCILSGCDYLSSLRGIGLAKACKVLRLANNPDIVKVIKKIGHYLKMNITVPEDYINGFIRANNTFLYQLVFDPIKRKLIPLNAYEDDVDPETLSYAGQYVDDSIALQIALGNKDINTFEQIDDYNPDTAMPAHSRSHSWDDKTCQKSANVSSIWHRNYSPRPESGTVSDAPQLKENPSTVGVERVISTKGLNLPRKSSIVKRPRSAELSEDDLLSQYSLSFTKKTKKNSSEGNKSLSFSEVFVPDLVNGPTNKKSVSTPPRTRNKFATFLQRKNEESGAVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREEACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLRLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UQ84 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S68 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| Y284 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| Y375 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| T475 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K482 | Acetylation | Uniprot | |
| K482 | Sumoylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| T502 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| T581 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | Uniprot | |
| S659 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| T732 | Phosphorylation | Uniprot | |
| K743 | Methylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| T752 | Phosphorylation | Uniprot | |
| K784 | Sumoylation | Uniprot | |
| K796 | Ubiquitination | Uniprot | |
| S804 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| T824 | Phosphorylation | Uniprot |
研究背景
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
Nucleus.
Note: Colocalizes with PCNA to discrete nuclear foci in S-phase.
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
Interacts with the MLH1-PMS2 heterodimer via MLH1. Interacts with MSH3. Interacts with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. Interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. Interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5'-overhanging flap structures. Interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5'-overhanging flap structures.
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
研究領域
· Genetic Information Processing > Replication and repair > Mismatch repair.
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