製品: MRPL20 Antibody
カタログ: DF3663
タンパク質の説明: Rabbit polyclonal antibody to MRPL20
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 22 KD; 17kD(Calculated).
ユニプロット: Q9BYC9
RRID: AB_2836035

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
MRPL20 Antibody detects endogenous levels of total MRPL20.
RRID:
AB_2836035
引用形式: Affinity Biosciences Cat# DF3663, RRID:AB_2836035.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

39S ribosomal protein L20, mitochondrial; L20mt; MGC4779; MGC74465; Mitochondrial ribosomal protein L20; MRPL 20;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MVFLTAQLWLRNRVTDRYFRIQEVLKHARHFRGRKNRCYRLAVRTVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPALIGNLVKCQVELNRKVLADLAIYEPKTFKSLAALASRRRHEGFAAALGDGKEPEGIFSRVVQYH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Pig
90
Zebrafish
60
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BYC9 基板として

Site PTM Type Enzyme
K26 Ubiquitination
Y39 Phosphorylation
T45 Phosphorylation
K82 Ubiquitination
K100 Ubiquitination
Y108 Phosphorylation
K111 Ubiquitination
K114 Ubiquitination
S121 Phosphorylation

研究背景

細胞の位置付け:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. Interacts with OXA1L (By similarity).

タンパク質ファミリー:

Belongs to the bacterial ribosomal protein bL20 family.

研究領域

· Genetic Information Processing > Translation > Ribosome.

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