製品: RPS18 Antibody
カタログ: DF3679
タンパク質の説明: Rabbit polyclonal antibody to RPS18
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Zebrafish, Bovine, Sheep, Dog, Xenopus
分子量: 17 KD; 18kD(Calculated).
ユニプロット: P62269
RRID: AB_2836051

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
RPS18 Antibody detects endogenous levels of total RPS18.
RRID:
AB_2836051
引用形式: Affinity Biosciences Cat# DF3679, RRID:AB_2836051.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

40S ribosomal protein S18; HKE3; KE 3; Ke-3; Ke3; Rhabdomyosarcoma antigen MU-RMS-40.21; Ribosomal protein S18; rps18; RS18_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human RPS18, corresponding to a region within C-terminal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Horse
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62269 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K8 Acetylation
K8 Ubiquitination
R23 Methylation
K25 Ubiquitination
K34 Ubiquitination
Y40 Phosphorylation
R46 Methylation
K47 Ubiquitination
K54 Acetylation
K54 Ubiquitination
R55 Methylation
T60 Phosphorylation
K78 Acetylation
K78 Sumoylation
K78 Ubiquitination
K88 Sumoylation
K88 Ubiquitination
K94 Acetylation
K94 Sumoylation
K94 Ubiquitination
Y95 Phosphorylation
S96 Phosphorylation
K106 Acetylation
K106 Sumoylation
K106 Ubiquitination
R113 Methylation

研究背景

機能:

Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the universal ribosomal protein uS13 family.

研究領域

· Genetic Information Processing > Translation > Ribosome.

参考文献

1). Identification of Bone Morphometric Protein-Related Hub Genes and Construction of a Transcriptional Regulatory Network in Patients With Ossification of the Ligamentum Flavum. Spine, 2023 (PubMed: 37384872) [IF=3.0]

Application: WB    Species: Human    Sample:

Figure 8 Protein levels of ADIPOQ, SCD, WDR82, and SPON1 were significantly downregulated, whereas those of SCX and RPS18 were significantly upregulated in the OLF group compared with the non-OLF group. *P < 0.05, **P < 0.01. CON indicates Nonossified ligamentum flavum; OLF, ossification of the ligamentum flavum.

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.