ARFGAP1 Antibody - #DF3747
| 製品: | ARFGAP1 Antibody |
| カタログ: | DF3747 |
| タンパク質の説明: | Rabbit polyclonal antibody to ARFGAP1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Rabbit |
| 分子量: | 60/48 KD; 45kD(Calculated). |
| ユニプロット: | Q8N6T3 |
| RRID: | AB_2836111 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3747, RRID:AB_2836111.
折りたたみ／展開
ADP ribosylation factor 1 GTPase activating protein; ADP ribosylation factor GTPase activating protein 1; ADP-ribosylation factor 1 GTPase-activating protein; ADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ARF1 directed GTPase activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein; ARF1GAP; ARFG1_HUMAN; Arfgap1; GAP protein; HRIHFB2281;
免疫原
- Q8N6T3 ARFG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8N6T3 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S57 | Phosphorylation | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K74 | Acetylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| T189 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| K226 | Acetylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K231 | Acetylation | Uniprot | |
| K231 | Sumoylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K263 | Acetylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| K297 | Sumoylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| Y311 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| C351 | S-Nitrosylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot |
研究背景
GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).
Cytoplasm. Golgi apparatus.
Note: Associates with the Golgi complex.
Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. The interaction with TMED2 inhibits the GAP activity (By similarity).
The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes (By similarity).
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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