AKR1A1 Antibody - #DF3752
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3752, RRID:AB_2836116.
折りたたみ／展開
AK1A1_HUMAN; Akr1a1; Alcohol dehydrogenase [NADP+]; Alcohol dehydrogenase; Aldehyde reductase; Aldo keto reductase family 1 member A1 (aldehyde reductase); Aldo-keto reductase family 1 member A1; ALDR1; ALR; ARM; DD3; MGC12529; MGC1380;
免疫原
Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung.
- P14550 AK1A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASCVLLHTGQKMPLIGLGTWKSEPGQVKAAVKYALSVGYRHIDCAAIYGNEPEIGEALKEDVGPGKAVPREELFVTSKLWNTKHHPEDVEPALRKTLADLQLEYLDLYLMHWPYAFERGDNPFPKNADGTICYDSTHYKETWKALEALVAKGLVQALGLSNFNSRQIDDILSVASVRPAVLQVECHPYLAQNELIAHCQARGLEVTAYSPLGSSDRAWRDPDEPVLLEEPVVLALAEKYGRSPAQILLRWQVQRKVICIPKSITPSRILQNIKVFDFTFSPEEMKQLNALNKNWRYIVPMLTVDGKRVPRDAGHPLYPFNDPY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P14550 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| C134 | S-Nitrosylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| Y325 | Phosphorylation | Uniprot |
研究背景
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde. Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Displays no reductase activity towards retinoids (By similarity).
Cytoplasm>Cytosol. Apical cell membrane.
Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung.
Monomer.
Belongs to the aldo/keto reductase family.
研究領域
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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